Life Technologies
Senior Staff Scientist at Life Technologies
Skills:
Genomics Lifesciences Polymers Biochemistry Assay Development Molecular Biology Qpcr Dna Sequencing Pcr Biotechnology Protein Chemistry Drug Discovery Sequencing Dna High Throughput Screening
Us Patents
Optically-Detectable Enzyme Substrates And Their Method Of Use
Schuyler Corry - Eugene OR, US William Downey - Eugene OR, US Brian Filanoski - Spokane Valley WA, US Kyle Gee - Springfield OR, US Lawrence Greenfield - Eugene OR, US James Hirsch - Springfield OR, US Iain Johnson - Eugene OR, US Aleksey Rukavishnikov - Eugene OR, US
Assignee:
Life Technologies Corporation - Carlsbad CA
International Classification:
C12Q 1/34
US Classification:
435 18, 435227, 435231
Abstract:
The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula:.
Optically-Detectable Enzyme Substrates And Their Method Of Use
Schuyler Corry - Eugene OR, US William Downey - Eugene OR, US Brian Filanoski - Eugene OR, US Kyle Gee - Springfield OR, US I. Greenfield - Eugene OR, US James Hirsch - Springfield OR, US Iain Johnson - Eugene OR, US Aleksey Rukavishnikov - Eugene OR, US
The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
Site-Specific Labeling Of Affinity Tags In Fusion Proteins
Kyle Gee - Springfield OR, US Courtenay Hart - Eugene OR, US Wai-Yee Leung - Eugene OR, US Wayne Patton - Eugene OR, US Aleksey Rukavishnikov - Eugene OR, US Richard Haugland - Eugene OR, US Zhenjun Diwu - Sunnyvale CA, US
International Classification:
C12Q001/00 G01N033/00
US Classification:
435004000, 436086000
Abstract:
The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.
Fluorogenic Ph Sensitive Dyes And Their Method Of Use
Daniel Beacham - Eugene OR, US Jeffrey Dzubay - Eugene OR, US Kyle Gee - Springfield OR, US Vladimir Martin - Eugene OR, US Aleksey Rukavishnikov - Eugene OR, US
A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I:
Optically-Detectable Enzyme Substrates And Their Method Of Use
Schuyler Boon CORRY - Eugene OR, US William Louis Downey - Eugene OR, US Brian Filanoski - Spokane Valley WA, US Kyle Richard Gee - Springfield OR, US I. Lawrence Greenfield - Eugene OR, US James David Hirsch - Springfield OR, US Iain Johnson - Eugene OR, US Aleksey Rukavishnikov - Eugene OR, US
Assignee:
INVITROGEN CORPORATION - Carlsbad CA
International Classification:
G01N 33/53 C07D 501/60 C07D 498/04 C12Q 1/34
US Classification:
435 772, 540222, 540225, 540300, 435 18
Abstract:
The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
Site-Specific Labeling Of Affinity Tags In Fusion Proteins
Kyle Gee - Springfield OR, US Courtenay Hart - Eugene OR, US Wai-Yee Leung - San Ramon CA, US Wayne Patton - Newton MA, US Aleksey Rukavishnikov - Eugene OR, US Richard Haugland - Olympia WA, US Zhenjun Diwu - Okemos MI, US
Assignee:
INVITROGEN CORPORATION - Carlsbad CA
International Classification:
G01N 1/30 C07F 5/02 C07D 311/06
US Classification:
435 405, 548405, 549289, 549283
Abstract:
The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.
Site-Specific Labeling Of Affinity Tags In Fusion Proteins
Kyle GEE - Springfield OR, US Courtenay Hart - Eugene OR, US Wai-Yee Leung - San Ramon CA, US Wayne Patton - Newton MA, US Aleksey Rukavishnikov - Eugene OR, US Richard Haugland - Olympia WA, US Zhenjun Diwu - Okemos MI, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
G01N 21/76 C07F 5/02 C40B 70/00 C07D 311/18
US Classification:
506 41, 549288, 548405, 436 86
Abstract:
The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.