MOLECULAR BIOLOGY. DNA/RNA isolation, quantitative PCR, RT-PCR, site-directed mutagenesis, DNA sequencing, genotyping, recombinant DNA cloning. Protein tagging, protein engineering, transgenic expression, protein affinity chromatography, immunoprecipitation, electrophoresis, staining, Western blot, and mass spectrometry analysis. CELLULAR BIOLOGY. Mammalian and Drosophila tissue culture and cell culture, transfection. Immunohistochemistry, RNA interference (RNAi), ELISA, luciferase reporter expression assays, proliferation assays, apoptosis assays, cancer-related signal transduction pathway analysis. MICROSCOPY. Brightfield, fluorescence, laser scanning confocal microscopy, laser spinning disk confocal microscopy, genetically-encoded calcium indicator imaging with microfluidics technology. Scanning and transmission electron microscopy, image processing, analysis, and structural modeling. BIOINFORMATICS. DNA and protein sequence analysis and alignments, genomic and proteomic data mining, data analysis, and biostatistical analysis. High-content, high-volume data and image processing and management. COMMUNICATION. Excellent written, oral, creative, interpersonal, organizational, and problem-solving skills. Experienced in writing, reviewing, and editing scientific articles and reviews, abstracts, grants, protocols, presentations and posters. COMPUTER. Strong computer skills and software proficiency: Microsoft Office, Adobe Creative Suite, EndNote, PASW/SPSS Statistics, microscopy, ImageJ. Platforms: Windows, Macintosh, Linux.