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David B Doroquez

age ~44

from Reading, MA

Also known as:
  • David Doroquez
  • Dave Doroquez
  • David Z
  • David S
  • David Bagon Doroquez

David Doroquez Phones & Addresses

  • Reading, MA
  • 213 Highland Ave, Somerville, MA 02143 • 6176297618
  • 45 Loomis St, Malden, MA 02148 • 7813224026
  • Fremont, CA
  • Bedford, MA
  • Cambridge, MA

Work

  • Company:
    Brandeis university, dept. of biology
    2007
  • Position:
    Pkd foundation postdoctoral fellow / visiting scientist

Education

  • School / High School:
    MASSACHUSETTS INSTITUTE OF TECHNOLOGY- Cambridge, MA
    2000
  • Specialities:
    Ph.D. in Developmental Biology

Skills

MOLECULAR BIOLOGY. DNA/RNA isolation • quantitative PCR • RT-PCR • site-directed mutagenesis • DNA sequencing • genotyping • recombinant DNA cloning. Protein tagging • protein engineering • transgenic expression • protein affinity chromatography • immunoprecipitation • electrophoresis • staining • Western blot • and mass spectrometry analysis. CELLULA... • transfection. Immunohistochemistry • RNA interference (RNAi) • ELISA • luciferase reporter expression assays • proliferation assays • apoptosis assays • cancer-related signal transduction pathw... • fluorescence • laser scanning confocal microscopy • laser spinning disk confocal microscopy • genetically-encoded calcium indicator im... • image processing • analysis • and structural modeling. BIOINFORMATICS... • genomic and proteomic data mining • data analysis • and biostatistical analysis. High-content • high-volume data and image processing an... • oral • creative • interpersonal • organizational • and problem-solving skills. Experienced ... • reviewing • and editing scientific articles and revi... • abstracts • grants • protocols • presentations and posters. COMPUTER. St... • Adobe Creative Suite • EndNote • PASW/SPSS Statistics • microscopy • ImageJ. Platforms: Windows • Macintosh • Linux.

Resumes

David Doroquez Photo 1

David Doroquez Boston, MA

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Work:
BRANDEIS UNIVERSITY, Dept. of Biology

2007 to 2000
PKD Foundation Postdoctoral Fellow / Visiting Scientist
BRANDEIS UNIVERSITY, Dept. of Biology
Waltham, MA
Jun 2012 to Jul 2012
Course Instructor
MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Dept. of Biology and Whitehead Institute
Cambridge, MA
2000 to 2007
NSF Graduate Research Fellow
MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Dept. of Biology
Cambridge, MA
Sep 2002 to May 2004
Teaching Assistant
SANTA CLARA UNIVERSITY, Biology Department
Santa Clara, CA
1998 to 2000
Research Associate
SANTA CLARA UNIVERSITY, Dept. of Biology
Santa Clara, CA
Apr 1998 to Jun 1998
Teaching Assistant
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO, Dept. of Anesthesia
San Francisco, CA
1997 to 1997
Research Assistant
Education:
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Cambridge, MA
2000 to 2007
Ph.D. in Developmental Biology
SANTA CLARA UNIVERSITY
Santa Clara, CA
1995 to 1999
B.S. in Biology
Skills:
MOLECULAR BIOLOGY. DNA/RNA isolation, quantitative PCR, RT-PCR, site-directed mutagenesis, DNA sequencing, genotyping, recombinant DNA cloning. Protein tagging, protein engineering, transgenic expression, protein affinity chromatography, immunoprecipitation, electrophoresis, staining, Western blot, and mass spectrometry analysis. CELLULAR BIOLOGY. Mammalian and Drosophila tissue culture and cell culture, transfection. Immunohistochemistry, RNA interference (RNAi), ELISA, luciferase reporter expression assays, proliferation assays, apoptosis assays, cancer-related signal transduction pathway analysis. MICROSCOPY. Brightfield, fluorescence, laser scanning confocal microscopy, laser spinning disk confocal microscopy, genetically-encoded calcium indicator imaging with microfluidics technology. Scanning and transmission electron microscopy, image processing, analysis, and structural modeling. BIOINFORMATICS. DNA and protein sequence analysis and alignments, genomic and proteomic data mining, data analysis, and biostatistical analysis. High-content, high-volume data and image processing and management. COMMUNICATION. Excellent written, oral, creative, interpersonal, organizational, and problem-solving skills. Experienced in writing, reviewing, and editing scientific articles and reviews, abstracts, grants, protocols, presentations and posters. COMPUTER. Strong computer skills and software proficiency: Microsoft Office, Adobe Creative Suite, EndNote, PASW/SPSS Statistics, microscopy, ImageJ. Platforms: Windows, Macintosh, Linux.

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David Doroquez


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