Liu Y Shen

US Patent:

20060101539, May 11, 2006

Filed:

Nov 4, 2005

Appl. No.:

11/266880

Inventors:

William Ainley - Carmel IN, US
Cory Leatherman Larsen - Zionsville IN, US
Min Lu - Carmel IN, US
Liu Shen - Westfield IN, US
Pon Jayakumar - Carmel IN, US
Robbi Garrison - Fillmore IN, US
Dayakar Pareddy - Carmel IN, US
Jeffrey Beringer - Carmel IN, US

International Classification:

C12N 5/04
C12N 15/82
A01H 1/00

US Classification:

800278000, 435419000

Abstract:

The present invention relates to methods for the cryopreservation of transformed and non-transformed cells. Also provided by the subject invention are methods of recovering cells that have been cryopreserved. Cultures of cells that have been successfully recovered from cryopreservation are also provided.

US Patent:

20080102079, May 1, 2008

Filed:

Oct 26, 2007

Appl. No.:

11/925300

Inventors:

William Ainley - Carmel IN, US
Janna Armstrong - Indianapolis IN, US
Krishna Madduri - Westfield IN, US
Donald Merlo - Carmel IN, US
Kelley Smith - Lebanon IN, US
Mark Thompson - Zionsville IN, US
Steven Webb - Westfield IN, US
Liu Shen - Westfield IN, US

Assignee:

Dow AgroSciences LLC - Indianapolis IN

International Classification:

A61K 39/00
A61P 43/00
C07K 14/00
C12N 15/11
C12P 21/04

US Classification:

424190100, 435069300, 530350000, 536023700

Abstract:

This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic (Cp) α-toxin protein (Mcpa). Utility of this vaccine was demonstrated by reduction of lesion severity in NE challenge trails, for example. Also disclosed herein are novel approaches for producing this vaccine in significant quantities. One exemplified approach involves producing NE vaccine (mutated alpha toxin) in bacterial expression systems, preferably utilizing the system, for commercial use in controlling NE in the poultry industry. The subject vaccines can be administered preferably to chickens in several different ways. A novel, Type VI alpha toxin from chicken isolates of Cp is also disclosed.

US Patent:

20130104257, Apr 25, 2013

Filed:

Oct 17, 2012

Appl. No.:

13/653602

Inventors:

Dow AgroSciences LLC - Indianapolis IN, US
Sean Michael Russell - Carmel IN, US
Liu Yin Shen - Westfield IN, US
Sivarama Reddy Chennareddy - West Lafayette IN, US
Jyoti Rout - Portland OR, US
Stephen Novak - Westfield IN, US

Assignee:

DOW AGROSCIENCES LLC - Indianapolis IN

International Classification:

C12N 15/82

US Classification:

800278, 4353201, 435468, 435419, 800298

Abstract:

Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues using a disclosed bidirectional promoter from or bidirectional constitutive promoter (BBCP). The constructs provided comprise at least one such bi-directional promoter linked to multiple gene expression cassettes, wherein each of the gene expression cassettes comprises at least one transgene. In some embodiments, the constructs and methods provided allow expression of genes between two and twenty.

US Patent:

20100261884, Oct 14, 2010

Filed:

Jun 23, 2010

Appl. No.:

12/821815

Inventors:

William M. Ainley - Carmel IN, US
Janna Armstrong - Indianapolis IN, US
Krishna Madduri - Westfield IN, US
Donald J. Merlo - Carmel IN, US
Kelley A. Smith - Lebanon IN, US
Mark A. Thompson - Zionsville IN, US
Steven R. Webb - Westfield IN, US
Liu Y. Shen - Westfield IN, US

Assignee:

Dow AgroSciences LLC - Indianapolis IN

International Classification:

C07K 14/33

US Classification:

530350

Abstract:

This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic (Cp) α-toxin protein (Mcpa). Utility of this vaccine was demonstrated by reduction of lesion severity in NE challenge trails, for example. Also disclosed herein are novel approaches for producing this vaccine in significant quantities. One exemplified approach involves producing NE vaccine (mutated alpha toxin) in bacterial expression systems, preferably utilizing the system, for commercial use in controlling NE in the poultry industry. The subject vaccines can be administered preferably to chickens in several different ways. A novel, Type VI alpha toxin from chicken isolates of Cp is also disclosed.

US Patent:

20180010200, Jan 11, 2018

Filed:

Sep 15, 2017

Appl. No.:

15/705447

Inventors:

- Indianapolis IN, US
W. Michael AINLEY - Carmel IN, US
Jayakumar P. SAMUEL - Carmel IN, US
Zehui CAO - Westfield IN, US
Liu SHEN - Westfield IN, US
Cristie M. DEWES - Indianapolis IN, US

International Classification:

C12Q 1/68

Abstract:

The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.

US Patent:

20150064708, Mar 5, 2015

Filed:

Sep 3, 2014

Appl. No.:

14/475968

Inventors:

- Indianapolis IN, US
W. Michael AINLEY - Carmel IN, US
Jayakumar P. SAMUEL - Carmel IN, US
Zehui CAO - Westfield IN, US
Liu Y. SHEN - Westfield IN, US
Cristie M. DEWES - Indianapolis IN, US

International Classification:

C12Q 1/68

US Classification:

435 612

Abstract:

The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.