Liangjing C Chen

US Patent:

8361754, Jan 29, 2013

Filed:

Sep 28, 2009

Appl. No.:

12/568364

Inventors:

Liangjing Chen - Austin TX, US
Robert A. Setterquist - Austin TX, US
Gary Latham - Austin TX, US

Assignee:

Applied Biosystems, LLC - Carlsbad CA

International Classification:

C12P 19/34

US Classification:

435 911, 435194, 435183

Abstract:

The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.

US Patent:

20050232934, Oct 20, 2005

Filed:

Apr 19, 2004

Appl. No.:

10/827498

Inventors:

Liangjing Chen - Austin TX, US
Robert Setterquist - Austin TX, US
Gary Latham - Austin TX, US

Assignee:

Ambion, Inc. - Austin TX

International Classification:

A61K039/21
C12N009/22
C07H021/04
C12N015/867

US Classification:

424188100, 435069300, 435456000, 435199000, 435325000, 536023200

Abstract:

The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.

US Patent:

20100243451, Sep 30, 2010

Filed:

Feb 16, 2010

Appl. No.:

12/706472

Inventors:

GARY J. LATHAM - AUSTIN TX, US
LIANGJING CHEN - AUSTIN TX, US
SACHIN SAH - AUSTIN TX, US

International Classification:

G01N 27/26
C12Q 1/68
C07H 21/04
B01D 57/02

US Classification:

204452, 435 6, 536 2433, 204450

Abstract:

This disclosure relates to methods of determining the presence and position of AGG or interruptor elements within a trinucleotide (for example, CGG) repeat region, and to methods of determining the number of repeats present in this region, by amplifying a set of products with a set of primers of which at least one comprises a portion of the CGG repeat region, and resolving the products to produce a representation of product size and abundance.

US Patent:

20120107824, May 3, 2012

Filed:

Oct 28, 2011

Appl. No.:

13/284328

Inventors:

Gary J. LATHAM - Austin TX, US
Liangjing Chen - Austin TX, US
Andrew Hadd - Austin TX, US
Sachin Sah - Austin TX, US
Ru Cao - Austin TX, US

International Classification:

C12Q 1/68
C07H 21/00

US Classification:

435 612, 536 231

Abstract:

Methods are provided for determining the methylation status of GC-rich templates. The methods include use of GC reference standards that allow simultaneous characterization of methylation status and CGG repeat length. The methods are useful for detecting genotypes associated with GC-rich repeats, including Fragile X Syndrome.

US Patent:

20220177949, Jun 9, 2022

Filed:

Dec 20, 2021

Appl. No.:

17/556387

Inventors:

- Menlo Park CA, US
Richard Andrew Blidner - Austin TX, US
Liangjing Chen - Austin TX, US

International Classification:

C12Q 1/686
C12N 9/12
C12Q 1/6844

Abstract:

The invention relates to methods of RNA amplification, including methods for the reverse transcription of cDNA from RNA using a thermostable reverse transcriptase. In a particular aspect, the methods are capable of linear amplification of an RNA template through multiple cycles of cDNA synthesis.

US Patent:

20200140923, May 7, 2020

Filed:

Jan 16, 2018

Appl. No.:

16/478705

Inventors:

- Austin TX, US
Richard Andrew Blidner - Austin TX, US
Liangjing Chen - Austin TX, US

International Classification:

C12Q 1/686
C12N 9/12
C12Q 1/6844

Abstract:

The invention relates to methods of RNA amplification, including methods for the reverse transcription of cDNA from RNA using a thermostable reverse transcriptase. In a particular aspect, the methods are capable of linear amplification of an RNA template through multiple cycles of cDNA synthesis.

US Patent:

20140248625, Sep 4, 2014

Filed:

Feb 26, 2014

Appl. No.:

14/191128

Inventors:

- Austin TX, US
Liangjing CHEN - Austin TX, US
Sachin SAH - Austin TX, US

Assignee:

Asuragen, Inc. - Austin TX

International Classification:

C12Q 1/68

US Classification:

435 612, 536 2433

Abstract:

This disclosure relates to methods of determining the presence and position of AGG or interruptor elements within a trinucleotide (for example, CGG) repeat region, and to methods of determining the number of repeats present in this region, by amplifying a set of products with a set of primers of which at least one comprises a portion of the CGG repeat region, and resolving the products to produce a representation of product size and abundance.