Amie J Strong

US Patent:

20120148621, Jun 14, 2012

Filed:

Jun 11, 2010

Appl. No.:

13/377746

Inventors:

Yichen Lu - Wellesley MA, US
Nicholas Kuchner - Framingham MA, US
Amie Strong - Framingham MA, US
Zhenhui Xu - Hainan, CN

Assignee:

VACCINE TECHNOLOGIES, INCORPORATED - Wellesley MA

International Classification:

A61K 39/00
A61K 39/29
A61K 39/12
A61K 39/21
A61K 39/245
A61P 31/06
A61P 31/20
A61P 31/22
A61P 31/18
A61P 31/16
A61P 31/14
A61K 39/39
A61K 39/04

US Classification:

4242081, 4241841, 4242261, 4242271, 4242281, 4242091, 4242181, 4242311, 4242481

Abstract:

The present invention is directed to a method for promoting or stimulating a cell-mediated immune response to an antigen, by administering a target antigen (such as a protein) with a transport factor that contains a fragment of a bipartite protein exotoxin, but not the corresponding protective antigen. Preferred transport factors include the protective antigen binding domain of lethal factor (LFn) from , consisting of amino acids 1-255, preferably a fragment of at least 80 amino acids that shows at least 80% homology to LFn, and a fragment of about 105 amino acids from the carboxy portion that does not bind PA. The target antigen can include any molecule for which it would be desirable to elicit a CMI response, including viral antigens and tumor antigens.