Anne-Marie M Mettus

US Patent:

6423828, Jul 23, 2002

Filed:

Jun 22, 1999

Appl. No.:

09/337280

Inventors:

James A. Baum - Doylestown PA
Amy Jelen Gilmer - Langhorne PA
Anne-Marie Light Mettus - Feasterville PA

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

C07K 1400

US Classification:

530350

Abstract:

Disclosed are novel synthetically-modified nucleic acid segments encoding -endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.

US Patent:

6468523, Oct 22, 2002

Filed:

Nov 2, 1998

Appl. No.:

09/184748

Inventors:

Anne-Marie Light Mettus - Feasterville PA
James A. Baum - Doylestown PA

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

A01N 6300

US Classification:

424 932, 424 93461, 514 12, 514 2, 530350, 530825

Abstract:

Disclosed is a novel Lepidopteran- and Coleopteran-active -endotoxin polypeptide, and compositions comprising the polypeptide, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise nucleic acid segments encoding the polypeptide. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species.

US Patent:

6541448, Apr 1, 2003

Filed:

May 11, 2001

Appl. No.:

09/853533

Inventors:

Barbara Isaac - St. Charles MO
Elysia K. Krieger - Kirkwood MO
Anne-Marie Light Mettus - Feasterville PA
Farhad Moshiri - Chesterfield MO

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

C07K 1400

US Classification:

514 2, 530350, 4242461

Abstract:

A novel gene encoding a Coleopteran inhibitory insecticidal crystal protein is disclosed. The protein, tIC851, is insecticidally active and provides plant protection from at least cotton boll weevil, , when applied to plants in an insecticidally effective composition.

US Patent:

6809078, Oct 26, 2004

Filed:

Oct 5, 2001

Appl. No.:

09/972175

Inventors:

James A. Baum - Doylestown PA
Amy Jelen Gilmer - Langhorne PA
Anne-Marie Light Mettus - Feasterville PA

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

A61K 3800

US Classification:

514 12, 530350

Abstract:

Disclosed are novel synthetically-modified nucleic acid segments encoding -endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.

US Patent:

6825006, Nov 30, 2004

Filed:

Jul 22, 2002

Appl. No.:

10/200522

Inventors:

James A. Baum - Doylestown PA
Amy Jelen Gilmer - Langhorne PA
Anne-Marie Light Mettus - Feasterville PA

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

C12P 2106

US Classification:

435 691, 4353201, 4352523, 435325, 536 231, 530350

Abstract:

Disclosed are novel synthetically-modified nucleic acid segments encoding -endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.

US Patent:

7022897, Apr 4, 2006

Filed:

Apr 10, 2002

Appl. No.:

10/120255

Inventors:

Anne-Marie Light Mettus - Feasterville PA, US
James A. Baum - Doylestown PA, US

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

A01H 5/00
A01H 1/00
C12N 15/32
C12N 1/21
C12N 15/82

US Classification:

800302, 800279, 800260, 536 2371, 424 932, 435 711, 435418, 43525231, 4353201, 4352523

Abstract:

Disclosed are novel polynucleotides encoding Lepidopteran- and Coleopteran-active δ-endotoxin polypeptides and compositions comprising the polypeptides. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise the novel nucleic acid segments encoding the δ-endotoxin polypeptides. Also disclosed are methods of identifying related polynucleotides encoding δ-endotoxin polypeptides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species.

US Patent:

7208656, Apr 24, 2007

Filed:

Dec 13, 2005

Appl. No.:

11/302357

Inventors:

Barbara Isaac - St. Charles MO, US
Elysia K. Krieger - Kirkwood MO, US
Anne-Marie Light Mettus - Feasterville PA, US
Farhad Moshiri - Chesterfield MO, US

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

A01H 5/00
C12P 21/06
C07H 17/00
C07K 14/00

US Classification:

800302, 800260, 800295, 435 691, 4353201, 4352523, 435325, 536 321, 530350

Abstract:

A novel gene encoding a Coleopteran inhibitory insecticidal crystal protein is disclosed. The protein, tIC851, is insecticidally active and provides plant protection from at least cotton boll weevil, , when applied to plants in an insecticidally effective composition.

US Patent:

7256017, Aug 14, 2007

Filed:

Nov 29, 2004

Appl. No.:

10/999188

Inventors:

James A. Baum - Doylestown PA, US
Amy Jelen Gilmer - Langhorne PA, US
Anne-Marie Light Mettus - Feasterville PA, US

Assignee:

Monsanto Technology LLC - St. Louis MO

International Classification:

C12P 21/06
C07K 14/00

US Classification:

435 691, 530350

Abstract:

Disclosed are novel synthetically-modified nucleic acid segments encoding δ-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the α helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.