Bob B Buchanan

US Patent:

6380372, Apr 30, 2002

Filed:

Mar 31, 2000

Appl. No.:

09/540014

Inventors:

Myeong-Je Cho - Alameda CA
Gregorio del Val - El Cerrito CA
Maxime Caillau - Verdun sur Garonne, FR
Peggy G. Lemaux - Moraga CA
Bob B. Buchanan - Berkeley CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07H 2102

US Classification:

536 231, 4353201, 435410, 4352521, 435 691, 435183, 800295, 800298, 800278, 530412

Abstract:

The present invention provides barley thioredoxin h nucleic acids and proteins. The barley thioredoxin h nucleic acid may be isolated or it may be an expression vector. The expression vector may be operably linked to a transcriptional regulatory sequence. The invention also provides for transgenic plants comprising recombinant barley thioredoxin h. The invention also provides methods of expressing and purifying barley thioredoxin h.

US Patent:

6555116, Apr 29, 2003

Filed:

Jan 27, 1999

Appl. No.:

09/238379

Inventors:

Bob B. Buchanan - Berkeley CA
Gregorio del Val - El Cerrito CA
Rosa M. Lozano - Madrid, ES
Joshua H. Wong - South San Francisco CA
Boihon C. Yee - Walnut Creek CA
Oscar L. Frick - San Francisco CA

Assignee:

Regents of the University of California - Oakland CA

International Classification:

A61K 3936

US Classification:

4242751, 4241851, 514 2, 514 12, 426541, 426542, 436 86, 436120, 436904, 435 25, 435 681, 530350, 530370, 530379

Abstract:

Thioredoxin, a small dithiol protein, is a specific reductant for allergenic proteins and particularly allergenic proteins present in pollen and animal and plant sources. All targeted proteins contain disulfide (SâS) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (SâS) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by lipoic acid chemical reductant. Skin tests carried out with sensitized dogs showed that treatment of the pollens with reduced thioredoxin prior to injection eliminated or decreased the allergenicity of the pollen. Studies showed increased digestion of the pollen proteins by pepsin following reduction by thioredoxin. Pollen proteins that have been reduced by thioredoxin are effective and safe immunotherapeutic agents for decreasing or eliminating an animals allergic reaction that would otherwise occur upon exposure to the non-reduced pollen protein.

US Patent:

6583271, Jun 24, 2003

Filed:

Nov 23, 1999

Appl. No.:

09/448109

Inventors:

Bob B. Buchanan - Berkeley CA
Karoly Kobrehel - Montpellier, FR
Boihon C. Yee - Walnut Creek CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

A23J 112

US Classification:

530372, 530350, 530374, 530375, 426 20, 426549, 435 25, 435189, 435191

Abstract:

Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase) from either or wheat germ. When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin. Moreover, the reduced form of the inhibitors showed increased susceptibility to heat and proteolysis by either subtilisin or a protease preparation from germinating wheat seeds.

US Patent:

6600021, Jul 29, 2003

Filed:

Nov 23, 1999

Appl. No.:

09/448111

Inventors:

Bob B. Buchanan - Berkeley CA
Karoly Kobrehel - Montpellier, FR
Boihon C. Yee - Walnut Creek CA
Joshua H. Wong - South San Francisco CA
Rosa Lozano - Madrid, ES
Jin-an Jiao - Miami FL
Sungho Shin - Taejon, KR

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07K 14415

US Classification:

530372, 530350, 530300, 530375, 435210, 435189

Abstract:

Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the, Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase) from either or wheat germ. When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin. Moreover, the reduced form of the inhibitors showed increased susceptibility to heat and proteolysis by either subtilisin or a protease preparation from germinating wheat seeds.

US Patent:

6610334, Aug 26, 2003

Filed:

Nov 23, 1999

Appl. No.:

09/447615

Inventors:

Bob B. Buchanan - Berkeley CA
Karoly Kobrehel - Montpellier, FR
Boihon C. Yee - Walnut Creek CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

A21D 1000

US Classification:

426 18, 426 19, 426 23, 426 27, 426 94, 426549

Abstract:

Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the, Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase) from either or wheat germ. When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin. Moreover, the reduced form of the inhibitors showed increased susceptibility to heat and proteolysis by either subtilisin or a protease preparation from germinating wheat seeds.

US Patent:

6642437, Nov 4, 2003

Filed:

Sep 30, 1998

Appl. No.:

09/164210

Inventors:

Peggy G. Lemaux - Moraga CA
Myeong-Je Cho - Alameda CA
Bob B. Buchanan - Berkeley CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 504

US Classification:

800287, 800278, 800320, 8003201, 8003203, 435 691, 435 697, 435 698, 435420, 435424, 435430, 4354301, 435431

Abstract:

Methods for producing proteins in plant seeds are disclosed. Expression of the protein is driven by a seed-specific promoter and the protein is preferably expressed as a fusion polypeptide that includes a signal peptide that causes the protein to accumulate in a subcellular compartment to protect the protein.

US Patent:

6677433, Jan 13, 2004

Filed:

Feb 7, 2001

Appl. No.:

09/779375

Inventors:

Bob B. Buchanan - Berkeley CA
Gregorio del Val - San Diego CA
Oscar L. Frick - San Francisco CA

Assignee:

The Regents of the University of California - Berkeley CA

International Classification:

C07K 1400

US Classification:

530350, 530365, 530370, 530378, 530345, 514 2, 426656, 426 34, 426541, 426574, 426549, 424 944

Abstract:

Disulfide proteins showed mitigated allergenicity and increased digestibility by pepsin following reduction by thioredoxin. The sulfhydryl groups newly formed on reduction by thioredoxin (at 4Â C. ) or dithiothreitol (DTT) (at 55Â C. ) were blocked with a physiological disulfide, such as cystamine or oxidized glutathione (GSSG) to obtain stable forms of the disarmed allergen. When derivatized with cystamine, BLG was separated from its oxidized and reduced forms on non-reducing SDS-PAGE and appeared to lack sulfhydryl groups. Although less effective GSSG, gave similar results. Allergenicity of the two derivatives was compared with that of the oxidized, reduced and reoxidized forms of BLG by skin testing dogs from a colony sensitized to cows milk. Both the cystamine and GSSG derivatized BLG showed decreased allergenicity and increased sensitivity to pepsin as compared to controls. The reoxidized form resembled the derivatives in having lower allergenicity.

US Patent:

6784346, Aug 31, 2004

Filed:

Mar 29, 2000

Appl. No.:

09/538864

Inventors:

Myeong-Je Cho - Alameda CA
Peggy G. Lemaux - Moraga CA
Bob B. Buchanan - Berkeley CA
Joshua Wong - San Francisco CA
Corina Marx - Oakland CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

A01H 500

US Classification:

800320, 800295, 8003203, 800278, 800287

Abstract:

Compositions and methods of use are provided herein to make and use transgenic plants with value-added traits.