Boris M Afanasiev

age ~71

from Loveland, CO

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Also known as:

Connected to:

Aedes Aegypti Densovirus Expression System
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US Patent:

58495231, Dec 15, 1998
Filed:

Jun 7, 1995
Appl. No.:

8/485341
Inventors:

Boris N. Afanasiev - Fort Collins CO
Jonathan Ored Carlson - Fort Collins CO
Barry J. Beaty - Fort Collins CO
David R. Higgins - San Diego CA
Kelly J. Thibault - San Diego CA
Assignee:

Research Corporation Technologies, Inc. - Tucson AZ
International Classification:

C12P 2106
C12N 506
C12N 1586
US Classification:

435 691
Abstract:

The present invention is directed to use of Aedes aegypti densonucleosis virus (Aedes DNV or AeDNV) as a heterologous gene expression system. The invention described herein provides promoters from the Aedes aegypti genome such as the p. 05, p7 and p61 promoters to generate expression vectors with cloning sites for insertion of heterologous genes. The invention further provides expression vectors comprising an AeDNV promoter operably linked to a heterologous gene and insect host cells comprising the vectors of the invention. Methods of producing recombinant proteins utilizing the vectors of the invention are also provided. The present invention further provides a kit containing the vectors of the invention for production of a recombinant protein.

Aedes Aegypti Densovirus Expression System
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US Patent:

56270488, May 6, 1997
Filed:

Nov 4, 1994
Appl. No.:

8/334669
Inventors:

Boris N. Afanasiev - Fort Collins CO
Jonathan O. Carlson - Fort Collins CO
Barry Beaty - Fort Collins CO
David R. Higgins - San Diego CA
Kelly J. Thibault - San Diego CA
Assignee:

Research Corporation Technologies, Inc. - Tucson AZ
International Classification:

C12P 2106
C12N 506
C12N 1586
US Classification:

435 691
Abstract:

The present invention is directed to use of Aedes aegypti densonucleosis virus (Aedes DNV or AeDNV) as a heterologous gene expression system. The invention described herein provides promoters from the Aedes aegypti genome such as the p. 05, p7 and p61 promoters to generate expression vectors with cloning sites for insertion of heterologous genes. The invention further provides expression vectors comprising an AeDNV promoter operably linked to a heterologous gene and insect host cells comprising the vectors of the invention. Methods of producing recombinant proteins utilizing the vectors of the invention are also provided. The present invention further provides a kit containing the vectors of the invention for production of a recombinant protein.

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