Charles H Greef from 61182 Fir Crest Knl, Bend, OR 97702, age 66, Phone: 5413896776

Us Patents

Hydrazide Building Blocks And Hydrazide Modified Biomolecules
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US Patent:

7129229, Oct 31, 2006
Filed:

Aug 10, 2001
Appl. No.:

10/344092
Inventors:

Stefan Raddatz - Wiesbaden, DE
Jochen Müller-Ibeler - Diez, DE
Markus Schweitzer - Frankfurt am Main, DE
Christoph Brücher - Eschborn, DE
Norbert Windhab - Hofheim, DE
John R. Havens - Arlington MA, US
Thomas J. Onofrey - Poway CA, US
Charles H. Greef - Boulder CO, US
Daguang Wang - Vista CA, US
Assignee:

Nanogen Recognomics GmbH - Frankfurt am Main
International Classification:

A61K 31/66
C07F 9/02
US Classification:

514114, 558 70, 558166, 558162, 558185
Abstract:

This invention relates to attachment chemistries for binding macromolecules to a substrate surface or to other conjugation targets. More particularly, this invention relates to attachment chemistries involving branched or linear structures having one or more hydrazide attachment moieties for binding the macromolecules to a substrate surface, or for other conjugation reactions. Novel modifying reagents are provided for the introduction of protected hydrazide attachment moieties or precursor forms of such hydrazides to the macromolecule, either as a single hydrazide or as multiple hydrazides.

Biomolecules Having Multiple Attachment Moieties For Binding To A Substrate Surface
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US Patent:

7186813, Mar 6, 2007
Filed:

Aug 11, 2000
Appl. No.:

10/181207
Inventors:

Markus Schweitzer - Frankfurt am Main, DE
Norbert Windhab - Hofheim am Taunus, DE
John R. Havens - Arlington MA, US
Thomas J. Onofrey - Poway CA, US
Charles Greef - Boulder CO, US
Daguang Wang - Carlsbad CA, US
Assignee:

Nanogen Recognomics GmbH - Frankfurt
International Classification:

C07H 21/00
C07H 21/02
C07H 21/04
C12Q 1/68
US Classification:

536 231, 536 253, 536 2533, 435 6
Abstract:

Biomolecules are provided having multiple binding sites for attachment to a substrate surface. The multiple attachment sites may be produced directly on the biomolecule or through use of branched phosphoramidite moieties that can be added in multiple to form dendritic structures which can in turn provide attachment sites for substrate binding moieties. Substrate binding moieties may include noncovalent binding moieties. For covalent binding moieties oligonucleotides containing hydrazides are provided. These hydrazides can be introduced via protected building blocks such as phosphoramidites or via building blocks containing precursor forms of such hydrazides.

Biomolecules Having Multiple Attachment Moieties For Binding To A Substrate Surface
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US Patent:

7833715, Nov 16, 2010
Filed:

Jan 18, 2007
Appl. No.:

11/655009
Inventors:

Markus Schweitzer - Frankfurt am Main, DE
Norbert Windhab - Hofheim am Taunus, DE
John R. Havens - Arlington MA, US
Thomas J. Onofrey - Poway CA, US
Charles Greef - Boulder CO, US
Daguang Wang - Vista CA, US
Assignee:

Nanogen Recognomics GmbH - Frankfurt am Main
International Classification:

C12Q 1/68
C07H 21/00
C07H 21/02
US Classification:

435 6, 536 231, 536 243, 536 253
Abstract:

Methods of binding biomolecules to a substrate are provided that include contacting the biomolecule with a branched linking moiety to form a branched linking structure. The branched linking structure is then contacted with a binding moiety on the substrate to form a coupled substrate binding structure, thereby binding the biomolecule to the substrate. The biomolecule may contain a Lewis base or a nucleophile to react with a Lewis acid or electrophile in the branched linking moiety. Alternatively, the biomolecule may contain a Lewis acid or electrophile that can react with a Lewis base or nucleophile in the branched linking moiety. Additionally, the biomolecule can be bound to the substrate through a covalent or non-covalent bond.

Biomolecules Having Multiple Attachment Moieties For Binding To A Substrate Surface
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US Patent:

8067581, Nov 29, 2011
Filed:

Nov 15, 2010
Appl. No.:

12/946777
Inventors:

Markus Schweitzer - Frankfurt am Main, DE
Norbert Windhab - Hofheim am Taunus, DE
John R. Havens - Arlington MA, US
Thomas J. Onofrey - Poway CA, US
Charles Greef - Boulder CO, US
Daguang Wang - Vista CA, US
Assignee:

Sanofi-Aventis S.A.
International Classification:

C07H 19/04
C07H 21/02
C07K 2/00
C07K 16/00
C12Q 1/68
US Classification:

536 266, 536 231, 435 6, 530300, 5303911
Abstract:

Compounds relating to attachment chemistries for binding biomolecules to a substrate surface are described. These include compounds of the following structure:.

Biomolecular Attachment Sites On Microelectronic Arrays And Methods Thereof
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US Patent:

8288155, Oct 16, 2012
Filed:

Jul 13, 2007
Appl. No.:

11/777919
Inventors:

John R. Havens - San Diego CA, US
Thomas J. Onofrey - San Marcos CA, US
Charles H. Greef - Ramona CA, US
Gregory J. Kevorkian - Temecula CA, US
Jain Krotz - San Diego CA, US
Kristie L. Lykstad - San Diego CA, US
Daniel E. Raymond - San Diego CA, US
Howard R. Reese - Poway CA, US
Regina Rooney - La Jolla CA, US
John J. Scott - Lafayette IN, US
Assignee:

Gamida for Life B.V. - Rotterdam
International Classification:

C12M 1/34
C12M 3/00
US Classification:

4352872, 4352831, 435 71, 436518, 436807
Abstract:

Methods of addressing a biomolecule to a selectively addressable electrode are described. A permeation layer overlying a plurality of selectively addressable electrodes is provided. The permeation layer includes a reactive group that is adapted to bond to a biomolecule and that requires activation through a chemical transformation before bonding to the biomolecule. At least one selectively addressable electrode is biased such that a pH change occurs in an overlying solution of the at least one selectively addressable electrode. The reactive group in a portion of the permeation layer above the at least one selectively addressable electrode is then chemically transformed to an activated reactive group as a result of the pH change. A biomolecule is then bound to the permeation layer overlying the at least one selectively addressable electrode through the activated reactive group.

System And Method For Detecting Multiple Molecules In One Assay
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US Patent:

8586347, Nov 19, 2013
Filed:

Sep 15, 2011
Appl. No.:

13/233794
Inventors:

Michael J. Lochhead - Boulder CO, US
Jeffrey Ives - Arvada CO, US
Kathryn Todorof - Boulder CO, US
Charles Greef - Louisville CO, US
Marie J. Delaney - Boulder CO, US
Kevin D. Moll - Boulder CO, US
Kurt R. Vogel - Boulder CO, US
Keagan B. Rowley - Boulder CO, US
Evelyn S. Woodruff - Longmont CO, US
John S. Dunn - Arvada CO, US
Christopher J. Myatt - Boulder CO, US
Daniel T. Nieuwlandt - Longmont CO, US
Assignee:

MBio Diagnostics, Inc. - Boulder CO
International Classification:

C40B 30/04
G01N 21/75
C12Q 1/68
C12M 1/00
US Classification:

4352831, 435 61, 422 8205, 506 9, 506 39, 385 14
Abstract:

A rapid diagnostic system that delivers a panel of serologic assay results using a small amount of blood, serum, or plasma is described. The system includes a disposable cartridge and a reader instrument, based on planar waveguide imaging technology. The cartridge incorporates a microarray of recombinant antigens and antibody controls in a fluidic channel, providing multiple parallel fluorescence assay results for a single sample.

Inorganic Permeation Layer For Micro-Electric Device
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US Patent:

20020085954, Jul 4, 2002
Filed:

Oct 22, 2001
Appl. No.:

10/029472
Inventors:

John Havens - San Diego CA, US
Michael Krihak - San Diego CA, US
Charles Greef - Ramona CA, US
Daniel Raymond - San Diego CA, US
Michael Heller - Encinitas CA, US
Assignee:

Nanogen, Inc. - San Diego CA
International Classification:

C12M001/34
B32B005/02
US Classification:

422/063000, 435/287200
Abstract:

The present invention pertains to a method of, and a device created by, depositing an inorganic permeation layer on a micro-electronic device for molecular biological reactions. The permeation layer is preferably sol-gel. The sol-gel permeation layer can be created with pre-defined porosity, pore size distribution, pore morphology, and surface area. The sol-gel permeation layer may also function as the attachment layer of the micro-electric device.

System And Method For Detection And Analysis Of A Molecule In A Sample
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US Patent:

20120316077, Dec 13, 2012
Filed:

Jun 7, 2012
Appl. No.:

13/491380
Inventors:

Charles Greef - Louisville CO, US
Michael J. Lochhead - Boulder CO, US
International Classification:

G01N 21/64
C40B 30/04
G01N 33/53
US Classification:

506 9, 436501
Abstract:

Disclosed here is a system and method for analyzing and/or detecting one or more target analytes in a sample by using a competitive assay. A standard curve may be constructed using known amounts of a molecule that is identical or substantially identical to the target analyte. Signals obtained from the target analyte can be compared against the standard curve in order to determine the level of the target analyte in the sample. The disclosed methods may be used in a multiplexed analyte detection and quantitation system.