Chenming Ming Zhang

Address:

304 Vinyard Ave, Blacksburg, VA 24060

VIN:

5FNRL38287B000720

Make:

HOND

Model:

ODYS

Year:

2007

US Patent:

20020055145, May 9, 2002

Filed:

Nov 30, 2001

Appl. No.:

09/998619

Inventors:

Gregory Conn - Cary NC, US
Brian Reardon - Seattle WA, US
Xianfang Zeng - Northborough MA, US
Chenming Zhang - Blacksburg VA, US

Assignee:

Diosynth RTP, Inc.

International Classification:

C12P021/02
C07K001/16

US Classification:

435/069100, 530/417000

Abstract:

The invention is directed to methods for purifying Troponin I, particularly recombinant Tropnin I produced in a bacterial expression system. Recombinant Tropnin I can be advantageously purified after reversibly protecting the free sulfhydryl groups, e.g., by forming sulfates. In a specific example, Tropnin I reacted with sodium tetrafhionate yielded sulfitolyzed Tropnin I, which was purified by chromatography on an anion exchanger, followed by hydrophobic interaction chromatography. Facile deprotection of the sulfhydryl groups yields a highly purified product ready for refolding.

US Patent:

20020064835, May 30, 2002

Filed:

Jul 10, 2001

Appl. No.:

09/903398

Inventors:

Gregory Conn - Cary NC, US
Brian Reardon - Seattle WA, US
Xianfang Zeng - Northborough MA, US
Chenming Zhang - Blacksburg VA, US

Assignee:

Diosynth RTP, Inc.

International Classification:

C12P021/04
A61K038/00

US Classification:

435/071200, 514/002000

Abstract:

The invention is directed to methods for purifying Troponin I, particularly recombinant Tropnin I produced in a bacterial expression system. Recombinant Tropnin I can be advantageously purified after reversibly protecting the free sulfhydryl groups, e.g., by forming sulfates. In a specific example, Tropnin I reacted with sodium tetrafhionate yielded sulfitolyzed Tropnin I, which was purified by chromatography on an anion exchanger, followed by hydrophobic interaction chromatography. Facile deprotection of the sulfhydryl groups yields a highly purified product ready for refolding.

US Patent:

20030105017, Jun 5, 2003

Filed:

Sep 26, 2002

Appl. No.:

10/255244

Inventors:

Gregory Conn - Cary NC, US
Brian Reardon - Seattle WA, US
Xianfang Zeng - Northborough MA, US
Chenming Zhang - Blacksburg VA, US

International Classification:

A61K038/17
C12P021/06
C07K014/47

US Classification:

514/012000, 530/350000, 435/069100

Abstract:

The invention is directed to methods for purifying Troponin I, particularly recombinant Tropnin I produced in a bacterial expression system. Recombinant Tropnin I can be advantageously purified after reversibly protecting the free sulfhydryl groups, e.g., by forming sulfates. In a specific example, Tropnin I reacted with sodium tetrafhionate yielded sulfitolyzed Tropnin I, which was purified by chromatography on an anion exchanger, followed by hydrophobic interaction chromatography. Facile deprotection of the sulfhydryl groups yields a highly purified product ready for refolding.

US Patent:

20030138907, Jul 24, 2003

Filed:

Nov 4, 2002

Appl. No.:

10/287118

Inventors:

Gregory Conn - Cary NC, US
Brian Reardon - Seattle WA, US
Xianfang Zeng - Northborough MA, US
Chenming Zhang - Blacksburg VA, US

International Classification:

C12P021/02
C12N001/21
C07K014/47
C07H021/04

US Classification:

435/069100, 435/252330, 435/320100, 530/350000, 536/023500

Abstract:

The invention is directed to methods for purifying Troponin I, particularly recombinant Troponin I produced in a bacterial expression system. Recombinant Troponin I can be advantageously purified after reversibly protecting the free sulfhydryl groups, e.g., by forming sulfates. In a specific example, Tropnin I reacted with sodium tetrafhionate yields sulfitolyzed Tropnin I, which was purified by chromatography on an anion exchanger, followed by hydrophobic interaction chromatography. Facile deprotection of the sulfhydryl groups yields a highly purified product ready for refolding.

US Patent:

20080274501, Nov 6, 2008

Filed:

May 2, 2008

Appl. No.:

12/114507

Inventors:

Chenming ZHANG - Blacksburg VA, US
Chris Holler - Lexington KY, US

International Classification:

C12P 21/00
C07K 14/00

US Classification:

435 691, 530412, 530421, 530417

Abstract:

The present invention provides a rapid and relatively simple process for purification of acidic proteins expressed in tobacco cells. The process comprises three main purification steps: precipitation with polyethyleneimine, column chromatography with a hydrophobic interaction resin, and column chromatography with hydroxyapatite. The process provides pure or essentially pure protein at a very high yield.

US Patent:

20190351037, Nov 21, 2019

Filed:

Jan 5, 2017

Appl. No.:

16/476252

Inventors:

Chenming ZHANG - Blacksburg VA, US
Zongmin ZHAO - Blacksburg VA, US
Yun HU - Blacksburg VA, US

International Classification:

A61K 39/00
A61K 39/385
A61K 9/51
A61P 25/34

Abstract:

Provided herein are nicotine polymer-stabilized nanoparticles, formulations thereof, and vaccines. Also provided herein are methods of treating and/or preventing nicotine addiction in a subject in need thereof.