Deborah P Freyler

US Patent:

49941925, Feb 19, 1991

Filed:

Jan 16, 1990

Appl. No.:

7/465835

Inventors:

Alan F. Corin - Rochester NY
Richard L. Columbus - Rochester NY
Deborah P. Freyler - Rochester NY

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

B01D 2126

US Classification:

210782

Abstract:

Apparatus and a method are described, wherein a coagulator accelerator is added to a container to achieve agglutination of blood cells when centrifuging whole blood, allowing the serum to be more easily poured off. The accelerator is selected from polymeric amines that are either polymerized amino acids or vinyl addition polymer amines, with a specified molecular weight range, the vinyl addition amines being selected to avoid lysing the blood cells.

US Patent:

50322885, Jul 16, 1991

Filed:

May 16, 1990

Appl. No.:

7/524401

Inventors:

Richard L. Columbus - Rochester NY
Susan M. Atwood - Newark NY
Deborah P. Freyler - Rochester NY
Harvey J. Palmer - Lima NY

Assignee:

Eastman Kodak Company - Rochester NY

International Classification:

B01D 2126

US Classification:

210741

Abstract:

A method of use is disclosed of a device that causes phase separation of whole blood, using much lower centrifugal forces. As a result, leukocytes are separated from blood cells having specific gravities of 1. 09 g/ml or higher. The method preferably uses a separation chamber arranged so that its long dimension or axis is parallel, not perpendicular, to the spin axis, and a valve that allows automatic removal of the ligher phase(s). In one aspect, the method separates a lighter phase from a heavier phase, for example, lymphocytes from whole blood, using a centrifugal force of reduced amount. In another aspect, DNA is extracted readily from the separated lighter phase since it is less likely that the lighter phase will be contaminated by erythrocytes as occurs in conventional separation techniques.