Delia E Penza

US Patent:

55275460, Jun 18, 1996

Filed:

Aug 10, 1994

Appl. No.:

8/288516

Inventors:

Delia E. Penza - Alamo CA
Susan K. Faris - San Francisco CA
Kenneth J. Lembach - Danville CA

Assignee:

Bayer Corporation - Berkeley CA

International Classification:

A61K 3512

US Classification:

424573

Abstract:

A previously undescribed Interleukin-6 inhibitor activity has been successfully isolated from the supernatant of the human promyelocytic leukemia cell line HL-60. Treatment of the HL-60 cell line with cycloheximide prevents the appearance of the inhibitory activity in the cellular supernatant. Incubation of the HL-60 supernatant with trypsin destroys the activity. The above observations indicate the inhibitor is a protein. Membrane and gel filtration studies indicate the protein has a molecular weight between 10,000 and 30,000 daltons. The inhibitor was partially isolated from other proteins by dye-ligand and reverse phase chromatography.