Edward J Sheldon

US Patent:

6375899, Apr 23, 2002

Filed:

Dec 2, 1998

Appl. No.:

09/203730

Inventors:

Donald E. Ackley - Cardiff CA
Edward L. Sheldon - San Diego CA
Michael K. Krihak - San Diego CA

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

G01N 100

US Classification:

422 681, 422 50, 422 63, 422 69, 422 70, 422 99, 422129, 4352831, 4352851, 4352852, 4352865, 4352873, 4352891, 4352941, 4353051, 4353052

Abstract:

Methods, apparatus, and applications for use of a stacked, reconfigurable system for electrophoretic transport are provided. In one embodiment, a system having a first chamber including at least a bottom support and an intermediate support, and a second chamber, said second chamber including a bottom support and a top member, the first and second chambers being coupled through a via. Electrophoretic, and optional electro-osmotic and thermal, transport is effected. In another aspect of this invention, three or more chambers are coupled by an electrophoretic buss. The electrophoretic buss includes driving electrodes and is adapted to receive fluid containing materials for transport. The chambers are coupled to the electrophoretic buss and serve as a tap from the buss for delivery of charged materials. In one embodiment, certain functions are performed in different chambers. For example, the first chamber may receive the sample and perform sample processing functions, the second chamber may perform amplification procedures, yet a third chamber may perform hybridization or other assays, and yet another chamber may perform immunoassays.

US Patent:

6468744, Oct 22, 2002

Filed:

Nov 17, 1999

Appl. No.:

09/341399

Inventors:

Maureen T. Cronin - Los Altos CA
Edward L. Sheldon - San Diego CA
Charles G. Miyada - San Jose CA
Earl A. Hubbell - Los Angeles CA
Mark Chee - Del Mar CA
Stephen P. A. Fodor - Palo Alto CA
Xiaohua C. Huang - Mountain View CA
Robert J. Lipshutz - Palo Alto CA
Peter E. Lobban - Los Altos CA
MacDonald S. Morris - Felton CA

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

The invention provides methods for detecting variations in polymorphic sites and/or variations in gene copy number. The methods are particularly useful for analysis of biotransformation genes, such as cytochromes P450.

US Patent:

6638482, Oct 28, 2003

Filed:

Dec 2, 1998

Appl. No.:

09/204324

Inventors:

Donald E. Ackley - Cardiff CA
Edward L. Sheldon - San Diego CA
Michael K. Krihak - San Diego CA

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

G01N 1500

US Classification:

422 681, 422 50, 422129, 435 6, 435 7, 435 911

Abstract:

Methods and apparatus for use of a stacked, reconfigurable system is provided. The stacked, reconfigurable system includes an inlet for receipt of a sample, a first chamber defined by a bottom support, an intermediate member, and a first spacer, the first chamber being coupled to the inlet through the bottom support, the first chamber including an analysis system having electrodes for electrophoretic transport of material. A second chamber is stacked on top of the first chamber, the second chamber being defined by the intermediate member, a top member, and a second spacer, the second chamber being in fluid communication with the first chamber through at least one via formed in the intermediate member, the second chamber being electrically reconfigurable to permit action such as, for example, synthesis of a compound or specialized analysis. The apparatus can be used to perform analysis on charged biological materials.

US Patent:

7115364, Oct 3, 2006

Filed:

Aug 2, 1995

Appl. No.:

08/510521

Inventors:

Mark Chee - Palo Alto CA, US
Maureen T. Cronin - Los Altos CA, US
Stephen P. A. Fodor - Palo Alto CA, US
Thomas R. Gingeras - Santa Clara CA, US
Xiaohua C. Huang - Mountain View CA, US
Earl A. Hubbell - Mountain View CA, US
Robert J. Lipshutz - Palo Alto CA, US
Peter E. Lobban - Palo Alto CA, US
Charles Garrett Miyada - Sunnyvale CA, US
Macdonald S. Morris - San Jose CA, US
Nila Shah - Saratoga CA, US
Edward L. Sheldon - San Diego CA, US

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 1/68
G01N 33/50
G01N 33/53
C07H 21/04

US Classification:

435 6, 422 50, 422 681, 536 243

Abstract:

The invention provides chips of immobilized probes, and methods employing the chips, for comparing a reference polynucleotide sequence of known sequence with a target sequence showing substantial similarity with the reference sequence, but differing in the presence of e. g. , mutations.

US Patent:

7399584, Jul 15, 2008

Filed:

Feb 22, 2000

Appl. No.:

09/510378

Inventors:

Maureen T. Cronin - Los Altos CA, US
Charles Garrett Miyada - San Jose CA, US
Earl A. Hubbell - Mountain View CA, US
Mark Chee - Palo Alto CA, US
Stephen P. A. Fodor - Palo Alto CA, US
Xiaohua C. Huang - Mountain View CA, US
Robert J. Lipshutz - Palo Alto CA, US
Peter E. Lobban - Palo Alto CA, US
MacDonald S. Morris - Felton CA, US
Edward L. Sheldon - San Diego CA, US

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 1/68
C12P 19/34
C12M 1/34
C07H 21/00

US Classification:

435 6, 435 911, 4352872, 536 231, 536 243

Abstract:

The invention provides methods of comparing a target nucleic acid with a reference nucleic acid using nucleic acid arrays.

US Patent:

7846659, Dec 7, 2010

Filed:

Apr 11, 2006

Appl. No.:

11/401482

Inventors:

Maureen T. Cronin - Los Altos CA, US
Charles G Miyada - San Jose CA, US
Earl A. Hubbell - Los Angeles CA, US
Mark Chee - Palo Alto CA, US
Stephen P. A. Fodor - Palo Alto CA, US
Xiaohua C. Huang - Mountain View CA, US
Robert J. Lipshutz - Palo Alto CA, US
Peter E. Lobban - Mountain View CA, US
MacDonald S. Morris - Felton CA, US
Edward L. Sheldon - San Diego CA, US

Assignee:

Affymetrix, Inc. - Santa Clara CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04

US Classification:

435 6, 536 231, 536 243, 536 2433

Abstract:

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequence of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the four corresponding probes from the four probe sets.

US Patent:

20010045359, Nov 29, 2001

Filed:

Jul 13, 2001

Appl. No.:

09/905755

Inventors:

Jing Cheng - San Diego CA, US
Edward Sheldon - San Diego CA, US
Lei Wu - San Diego CA, US
James O'Connell - San Diego CA, US

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

G01N027/26
G01N027/447
C12M001/14
C12N013/00

US Classification:

204/547000, 204/643000, 435/173100, 435/173700, 435/173900, 435/283100

Abstract:

The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge () including a microfabricated silicon chip () on a printed circuit board () and a flow cell () mounted to the chip () to form a flow chamber. The cartridge () also includes output pins () for electronically connecting the cartridge () to an electronic controller. The chip () includes a plurality of circular microelectrodes () which are preferably coated with a protective permeation layer. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.

US Patent:

20020155586, Oct 24, 2002

Filed:

Jun 5, 2002

Appl. No.:

10/163835

Inventors:

Jing Cheng - Bejing, CN
Lei Wu - San Diego CA, US
Michael Heller - Encinitas CA, US
Edward Sheldon - Arcadia CA, US
Jonathan Diver - San Diego CA, US
James O'Connell - Solana Beach CA, US
Dan Smolko - Jamul CA, US
Shila Jalali - San Diego CA, US
David Willoughby - San Diego CA, US

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

C12M001/34

US Classification:

435/287100, 435/287200, 435/288500

Abstract:

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.