William J. McAleer - Ambler PA Edward H. Wasmuth - Telford PA
Assignee:
Merck & Co., Inc. - Rahway NJ
International Classification:
A61K 3912 C12K 700
US Classification:
424 89
Abstract:
Biological fluid containing hepatitis B surface antigen is concentrated using ammonium sulfate, and subjected to an isopycnic banding followed by rate zonal banding.
William J. McAleer - Ambler PA Edward H. Wasmuth - Telford PA
Assignee:
Merck & Co., Inc. - Rahway NJ
International Classification:
A61K 3900 A61K 3912 A61K 3942
US Classification:
424 86
Abstract:
High purity HB. sub. s Ag is injected into guinea pigs to stimulate formulation of HB. sub. s Ab-positive serum. Gamma globulins containing HB. sub. s Ab are separated from the serum and the globulin fraction containing HB. sub. s Ab is isolated and coupled to a Sepharose gel. HB. sub. s Ag from HB. sub. s Ag-positive plasma is adsorbed on the gel by formation of an HB. sub. s Ab-HB. sub. s Ag complex. HB. sub. s Ag is eluted from the column and dialysed.
William J. McAleer - Ambler PA William J. Miller - North Wales PA Edward H. Wasmuth - Telford PA
Assignee:
Merck & Co., Inc. - Rahway NJ
International Classification:
A61K 3912 C12K 700
US Classification:
424 89
Abstract:
HB. sub. c Ag is prepared by isolating Dane particles by isopycnic banding of fluid from HB. sub. s Ag positive donors, optionally but preferably pelleting the Dane particles, and then removing the surface antigen by contacting the Dane particles with a nonionic surfactant having from about 15 to about 35 oxyethylene units in the presence of a reducing agent such as mercaptoethanol. The isolated core antigen is used as a diagnostic and immunologic agent.
Separating Antigen From Residual Common Sensitizing Protein
William J. McAleer - Ambler PA Edward H. Wasmuth - Telford PA
Assignee:
Merck & Co., Inc. - Rahway NJ
International Classification:
A61K 3912
US Classification:
424 89
Abstract:
An improved process for the preparation of hepatitis B antigen by subjecting biological fluid containing heptatitis B surface antigen to isopycanic banding wherein the step gradient is NaBr. Faster and more economical processing is obtained by multiple loading of the step gradient, either NaBr or conventional gradients.
William J. McAleer - Ambler PA Edward H. Wasmuth - Telford PA
Assignee:
Merck & Co., Inc. - Rahway NJ
International Classification:
A61K 3912 C12K 500 C07G 700
US Classification:
424 89
Abstract:
An improved process for the preparation of hepatitis B antigen by subjecting plasma of hepatitis B donors to isopycanic banding wherein the step gradient is NaBr. Faster and more economical processing is obtained by multiple loading of the step gradient, either NaBr or conventional gradients.
William J. McAleer - Ambler PA Edward H. Wasmuth - Telford PA
Assignee:
Merck & Co., Inc. - Rahway NJ
International Classification:
A61K 3912
US Classification:
424 89
Abstract:
Hepatitis B surface antigen free of gradient-derived added cesium and potassium ions, for example the antigen in an aqueous solution of sodium bromide.
William J. McAleer - Ambler PA Edward H. Wasmuth - Telford PA
Assignee:
Merck & Co., Inc. - Rahway NJ
International Classification:
A61K 3942
US Classification:
424 86
Abstract:
Hepatitis B immune globulin is prepared from individuals who exhibit an increase in hepatitis B surface antibody of at least 2,000 PHA units/ml following immunization with hepatitis B surface antigen.
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