Eugene P Stirchak

US Patent:

51420473, Aug 25, 1992

Filed:

Sep 23, 1987

Appl. No.:

7/100033

Inventors:

James Summerton - Corvallis OR
Dwight Weller - Corvallis OR
Eugene Stirchak - Corvallis OR

Assignee:

Anti-Gene Development Group - Corvallis OR

International Classification:

C07D41304
C07D41314

US Classification:

544118

Abstract:

A composition of polymer molecules effective to bind, with substantially uniform binding affinity, to a single-stranded polynucleotide containing a target sequence of bases. The polymer molecules are composed of a sequence of base-pairing moieties effective to hydrogen bond to corresponding, complementary bases in the target sequence, under selected binding conditions, and a predominantly uncharged, achiral backbone supporting the base-pairing moieties at positions and in orientations which allow hydrogen bonding between the pairing moieties of the polymer and the corresponding complementary bases in the target sequence. The composition has diagnostic uses, in a solid-support assay system, and therapeutic uses involving inhibition or inactivation of target polynucleotides.

US Patent:

52350339, Aug 10, 1993

Filed:

Dec 20, 1989

Appl. No.:

7/454056

Inventors:

James E. Summerton - Corvallis OR
Dwight D. Weller - Corvallis OR
Eugene P. Stirchak - Corvallis OR

Assignee:

Anti-Gene Development Group - Corvallis OR

International Classification:

C08G 7518
C08G 7520
C08G 7524
C07D41300

US Classification:

528391

Abstract:

Alpha-morpholino subunits and polymer compositions composed of alpha-morpholino subunits are disclosed. These subunits can be linked together by uncharged linkages, one to three atoms in length, joining the morpholino nitrogen of one subunit to the 5' exocyclic carbon of an adjacent subunit. Each subunit contains a purine or pyrimidine base-pairing moiety effective to bind by hydrogen bonding to a specific base or base-pair in a target polynucleotide. The polymers of the present invention can be used in place of standard RNA or DNA oligomers for a number of standard laboratory procedures, for example, as probes in solid-phase systems and nucleic acid hybridization analyses. Further, the polymers of the present invention have potential use as therapeutic agents in anti-sense-type technologies.

US Patent:

53788419, Jan 3, 1995

Filed:

Jun 8, 1993

Appl. No.:

8/074120

Inventors:

James E. Summerton - Corvallis OR
Dwight D. Weller - Corvallis OR
Eugene P. Stirchak - Corvallis OR

Assignee:

Antivirals Inc. - Corvallis OR

International Classification:

C07D41304
C07D47302
C07D47326

US Classification:

544118

Abstract:

Alpha-morpholino subunits and polymer compositions composed of alpha-morpholino subunits are disclosed. These subunits can be linked together by uncharged linkages, one to three atoms in length, joining the morpholino nitrogen of one subunit to the 5' exocyclic carbon of an adjacent subunit. Each subunit contains a purine or pyrimidine base-pairing moiety effective to bind by hydrogen bonding to a specific base or base-pair in a target polynucleotide. The polymers of the instant invention can be used in place of standard RNA or DNA oligomers. For example, the target-specific polymers of the invention can be used in a variety of diagnostic assays for detection of RNA or DNA having a given target sequence. Further, the polymers of the invention also have potential use as therapeutic agents.

US Patent:

58080430, Sep 15, 1998

Filed:

Jan 18, 1995

Appl. No.:

8/374456

Inventors:

R. Scott Duthie - Milwaukee WI
Charles K. Brush - Whitefish Bay WI
Eugene P. Stirchak - Brown Deer WI
Mark E. Freeman - Middlesex NJ
Lawrence J. Burazin - St. Francis WI

Assignee:

Pharmacia Biotech Inc. - Milwaukee WI

International Classification:

C07H 2100
C07H 1904

US Classification:

536 2532

Abstract:

A preparation of a labelled nucleotide comprising at least one compound having a Mg. sup. 2+ association constant between 1. times. 10. sup. -11 to 1. times. 10. sup. -2, inclusive. The compound is preferably selected from the group consisting of citrate, isocitrate, phosphate, EGTA, EDTA, and CDTA. The concentration of the compound is preferably at least 5 mM.

US Patent:

54709741, Nov 28, 1995

Filed:

Feb 25, 1994

Appl. No.:

8/202664

Inventors:

James Summerton - Corvallis OR
Dwight Weller - Corvallis OR
Eugene Stirchak - Corvallis OR

Assignee:

Neu-Gene Development Group - Corvallis OR

International Classification:

C07D41304
C07D41312
C07D47326

US Classification:

544118

Abstract:

A composition of polymer molecules effective to bind, with substantially uniform binding affinity, to a single-stranded polynucleotide containing a target sequence of bases. The polymer molecules are composed of a sequence of base-pairing moieties effective to hydrogen bond to corresponding, complementary bases in the target sequence, under selected binding conditions, and a predominantly uncharged, achiral backbone supporting the base-pairing moieties at positions and in orientations which allow hydrogen bonding between the pairing moieties of the polymer and the corresponding complementary bases in the target sequence. The composition has diagnostic uses, in a solid-support assay system, and therapeutic uses involving inhibition or inactivation of target polynucleotides.