Francis J Lenoch

US Patent:

61000396, Aug 8, 2000

Filed:

Jan 28, 1999

Appl. No.:

9/239355

Inventors:

Thomas J. Burke - Madison WI
Randall E. Bolger - Oregon WI
Francis J. Lenoch - Madison WI

Assignee:

Panvera, Inc. - Madison WI

International Classification:

C12Q 168
C07K 14005
C07H 2104

US Classification:

435 6

Abstract:

Described is a process for detecting reverse transcriptase activity and, thereby, reverse transcriptase inhibitors using fluorescence polarization, comprising, mixing a DNA primer with an RNA template. Then forming an RNA/DNA duplex utilizing the reverse transcriptase and removing the RNA from the RNA/DNA duplex to form single-stranded DNA. Finally, adding a fluorescent-labeled oligonucleotide complementary to the single-stranded DNA for hybridizing to the single-stranded DNA; and, measuring the fluorescence polarization.

US Patent:

58245570, Oct 20, 1998

Filed:

Apr 2, 1996

Appl. No.:

8/626520

Inventors:

Thomas J. Burke - Madison WI
Randall E. Bolger - Oregon WI
Francis J. Lenoch - Madison WI

Assignee:

PanVera Corporation - Madison WI

International Classification:

C12Q 168
G01N 3348

US Classification:

436 94

Abstract:

A homogeneous fluorescence-based nucleic acid detection and quantitation system is provided to measure nucleic acid in protein solutions. The process relies on the intercalation of a non-fluorescent dye into a double-stranded nucleic acid helix or single-stranded nucleic acid. The dye fluoresces after intercalation and the intensity is a direct measurement of the amount of nucleic acid present in the sample.