Herman A Berkhoff

US Patent:

47756217, Oct 4, 1988

Filed:

Feb 25, 1986

Appl. No.:

6/833881

Inventors:

Herman A. Berkhoff - Raleigh NC
Andrew C. Vinal - Raleigh NC

Assignee:

North Carolina State University - Raleigh NC

International Classification:

C12Q 110

US Classification:

435 38

Abstract:

A method for distinguishing invasive from noninvasive E. coli comprises collecting an undifferentiated bacterial sample from a host, contacting bacteria contained in the sample to a growth medium which contains a dye selectively taken up by invasive E. coli, incubating the growth medium to grow colonies of E. coli, and examining the growth medium to detect E. coli colonies which have taken up the dye. A two-step incubation procedure provides superior results. While the invention can be practiced with E. coli obtained from any host, specific procedures for practicing the invention in poultry are provided.