Jason G Knott

US Patent:

20050273870, Dec 8, 2005

Filed:

Jul 5, 2005

Appl. No.:

11/174825

Inventors:

James Robl - Beichertown MA, US
Jason Knott - Lancaster MA, US
Joseph Jerry - Shutasbury MA, US

International Classification:

A01K067/00
C12N015/85

US Classification:

800008000, 800021000, 435455000

Abstract:

The invention relates to a method of synchronizing a population of somatic cells in Gfor purposes of preparing the cells for nuclear transfer or nuclear transplantation by using mechanical shake-off and selection of mitotic doublet cells. This method may further comprise cooling of the cells or other means of synchronizing the cells in Gphase for longer periods of time. The invention also relates to the use of a synchronized population of rapidly, dividing somatic cells obtained by these methods as a source of donor nuclei or chromatin for use in nuclear transfer or nuclear transplantation.

US Patent:

20050025750, Feb 3, 2005

Filed:

Dec 29, 2003

Appl. No.:

10/747772

Inventors:

Rafael Fissore - Amherst MA, US
Jason Knott - Leicester MA, US
Manabu Kurokawa - Tokorozawa, JP

International Classification:

A61K048/00
C12N005/08

US Classification:

424093210, 435366000

Abstract:

The present invention provides a method for parthenogenetic activation by injection and subsequent removal of a sperm into a mammalian cell, for example, an oocyte, an embryo, a blastomere, an inner cell mass cell, or a morulae cell.