Jay M Shockey

US Patent:

7105722, Sep 12, 2006

Filed:

Apr 9, 2002

Appl. No.:

10/119136

Inventors:

Jay M. Shockey - Pullman WA, US
Judy Schnurr - Pullman WA, US
John A. Browse - Pullman WA, US

International Classification:

A01H 15/82
C12P 21/06

US Classification:

800281, 536 236, 435 691

Abstract:

The present invention relates to genes encoding plant acyl-CoA synthetases and methods of their use. In particular, the present invention is related to plant acyl-coenzyme A synthetases. The present invention encompasses both native and recombinant wild-type forms of the enzymes, as well as mutant and variant forms, some of which possess altered characteristics relative to the wild-type enzyme. The present invention also relates to methods of using acyl-CoA synthetases, including altered expression in transgenic plants and expression in prokaryotes and cell culture systems.

US Patent:

8101818, Jan 24, 2012

Filed:

May 22, 2006

Appl. No.:

11/915146

Inventors:

John A. Browse - Palouse WA, US
Jay M. Shockey - Mandeville LA, US
Julie Jeannine Burgal - Davis CA, US

Assignee:

Washington State University - Pullman WA

International Classification:

A01H 1/00
A01H 5/00
A01H 5/10
A01H 15/00
C07H 21/04
C12N 5/14
C12N 15/82

US Classification:

800278, 800281, 800306, 800312, 800314, 8003201, 800322, 536 236, 435410, 4353201, 435468, 4352541

Abstract:

Particular aspects provide six novel cDNA clones, including cloned sequences of: DGAT (RcDGAT1 and RcDGAT2); RcLPAT; LACS (RcLACS4), and PDAT (RcPDAT1A and RcPDAT1B). Additional aspects provide methods for substantially enhanced accumulation of hydroxy fatty acid (HFA) in transgenic plant tissue (e. g. , seeds), comprising expression of particular novel sequences. For example, expression of RcDGAT2 or RcPDAT1 in castor hydroxylase-expressing lines resulted in substantially enhanced accumulation of hydroxy fatty acid (HFA) (e. g. , to over 30%; a 50-70% increase in HFA accumulation) relative to the hydroxylase-only expressing parental lines. Further aspects provide methods to increase at least one of total lipid content, percent seed germination, and seed weight in transgenic plants, comprising expression of RcDGAT2 in castor hydroxylase-expressing plant lines. Yet further aspects provide methods for expressing and accumulating hydroxyl fatty acid in yeast (e. g. , TAG biosynthesis from diricinolein), comprising expression of RcDGAT2 RcDGAT2 coding sequences in yeast.

US Patent:

20030028915, Feb 6, 2003

Filed:

Jul 16, 2001

Appl. No.:

09/906408

Inventors:

Gregory Tilton - Pullman WA, US
Jay Shockey - Pullman WA, US
John Browse - Pullman WA, US

International Classification:

A01H001/00
C07H021/04
C12N015/82

US Classification:

800/278000, 435/320100, 536/023200

Abstract:

The present invention provides compositions and methods related to acyl-coenzyme A thioesterases. In particular, the present invention is related to plant acyl-coenzyme A thioesterases. The present invention encompasses both native and recombinant wild-type forms of the enzymes, as well as mutant and variant forms, some of which possess altered characteristics relative to the wild-type enzyme. The present invention also relates to methods of using acyl-CoA thioesterases, including altered expression in transgenic plants and expression in prokaryotes and cell culture systems.

US Patent:

20030037357, Feb 20, 2003

Filed:

Jul 16, 2001

Appl. No.:

09/906419

Inventors:

Jay Shockey - Pullman WA, US
Judy Schnurr - Pullman WA, US
John Browse - Pullman WA, US

Assignee:

Washington State University Research Foundation - Pullman WA

International Classification:

A01H005/00
C07H021/04
C12N015/82

US Classification:

800/278000, 435/320100, 536/023200

Abstract:

The present invention relates to genes encoding plant acyl-CoA synthetases and methods of their use. In particular, the present invention is related to plant acyl-coenzyme A synthetases. The present invention encompasses both native and recombinant wild-type forms of the enzymes, as well as mutant and variant forms, some of which possess altered characteristics relative to the wild-type enzyme. The present invention also relates to methods of using acyl-CoA synthetases, including altered expression in transgenic plants and expression in prokaryotes and cell culture systems.

US Patent:

20040010817, Jan 15, 2004

Filed:

Apr 9, 2003

Appl. No.:

10/410031

Inventors:

Jay Shockey - Mandeville LA, US
Judy Schnurr - Coon Rapids MN, US
John Browse - Palouse WA, US

Assignee:

Washington State University Research Foundation - Pullman WA

International Classification:

A01H001/00
C12N015/82
C07H021/04
C12N015/87
C12N005/04

US Classification:

800/281000, 435/069100, 435/419000, 435/193000, 435/320100, 536/023200

Abstract:

The present invention relates to genes encoding plant acyl-CoA synthetases and methods of their use. In particular, the present invention is related to plant acyl-coenzyme A synthetases. The present invention encompasses both native and recombinant wild-type forms of the enzymes, as well as mutant and variant forms, some of which possess altered characteristics relative to the wild-type enzyme. The present invention also relates to methods of using acyl-CoA synthetases, including altered expression in transgenic plants and expression in prokaryotes and cell culture systems.