Skills:
nano-ESI-LTQ MS-MS (Orbitrap Fusion), uHPLC MS-MS (Bruker triple quad), Peptide processing for MS and MS-MS (Trypsin digestion, Zip-Tip) MALDI-TOF MS and MALDI-TOF/TOF MS-MS, High Pressure Liquid Chromatography (HPLC), Bacterial, Mammalian, and Insect cell culture, Baculovirus expression system PCR, RT-PCR, Real Time qPCR Restriction Enzyme digests Agarose Gel Electrophoresis, SDS-Page-coomassie Gel Electrophoresis, Western Blot (ECL, BCIP-NBT) ELISA (Human-Rabbit-Mouse serum) MSD (Chicken, Goat, Rabbit serum) Southern blot, Gram stains, Transfection of cultured cells, Protein densitometry, Dialysis, Human Tissue Processing and Fixation, Solution Preparation, Aseptic techniques, Mouse surgeries, handling and injections (IP, IM, Tail injections)