Joaquin A Umana from 62 Summer Ave, Reading, MA 01867, age 59, Phone: 7819859634

Us Patents

Affinity Chromatography Matrices And Methods Of Making And Using The Same
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US Patent:

7846682, Dec 7, 2010
Filed:

Nov 13, 2009
Appl. No.:

12/590726
Inventors:

Nanying Bian - Nashua NH, US
John Charkoudian - Carlisle MA, US
Neil Soice - Amherst NH, US
Joaquin Umana - Stoneham MA, US
Chen Wang - Acton MA, US
Assignee:

Millipore Corporation - Billerica MA
International Classification:

G01N 33/53
US Classification:

435 71, 436518
Abstract:

The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

Affinity Chromatography Matrices And Methods Of Making And Using The Same
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US Patent:

8114611, Feb 14, 2012
Filed:

Nov 1, 2010
Appl. No.:

12/916978
Inventors:

Nanying Bian - Nashua NH, US
John Charkoudian - Carlisle MA, US
Neil Soice - Amherst NH, US
Joaquin Umana - Stoneham MA, US
Chen Wang - Acton MA, US
Assignee:

Millipore Corporation - Billerica MA
International Classification:

G01N 33/53
US Classification:

435 71, 435 72, 436518
Abstract:

The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

Affinity Chromatography Matrices And Methods Of Making And Using The Same
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US Patent:

20070207500, Sep 6, 2007
Filed:

Jan 5, 2007
Appl. No.:

11/650093
Inventors:

Nanying Bian - Nashua NH, US
John Charkoudian - Carlisle MA, US
Neil Soice - Amherst NH, US
Joaquin Umana - Stoneham MA, US
Chen Wang - Acton MA, US
Assignee:

Millipore Corporation - Billerica MA
International Classification:

G01N 33/53
C07K 14/47
US Classification:

435007100, 530412000, 502403000, 530333000
Abstract:

The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

Asymmetric Porous Adsorptive Bead
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US Patent:

20070212540, Sep 13, 2007
Filed:

Sep 13, 2006
Appl. No.:

11/520848
Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Georgetown NH, US
Chen Wang - Acton MA, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Joaquin Umana - Stoneham MA, US
Dennis Aquino - Chelmsford MA, US
Neil Soice - Amherst NH, US
Lyddiatt Andrew - Co Durham, GB
Assignee:

Millipore Corporation - Billerica MA
International Classification:

B32B 1/00
B01D 15/08
B01D 39/00
US Classification:

428402000, 210502100, 210198200
Abstract:

The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

Asymmertric Porous Adsorptive Bead
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US Patent:

20100174051, Jul 8, 2010
Filed:

Mar 2, 2010
Appl. No.:

12/660715
Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Nashua NH, US
Chen Wang - Acton MA, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Joaquin A. Umana - Stoneham MA, US
Dennis Aquino - Chelmsford MA, US
Neil Soice - Amherst NH, US
Lyddiatt Andrew - Co Durham, GB
Assignee:

Millipore Corporation - Billerica MA
International Classification:

C07K 1/22
US Classification:

5303881
Abstract:

The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

Method And Apparatus For Making Porous Agarose Beads
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US Patent:

20100227015, Sep 9, 2010
Filed:

Mar 2, 2010
Appl. No.:

12/660708
Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Nashua NH, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Joaquin Umana - Stoneham MA, US
Neil P. Soice - Amherst NH, US
Assignee:

Millipore Corporation - Billerica MA
International Classification:

B01J 13/04
US Classification:

425 5
Abstract:

The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads. An apparatus for running the process is also disclosed.

Chromatography Media And Method
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US Patent:

20120029176, Feb 2, 2012
Filed:

Jul 27, 2011
Appl. No.:

13/191992
Inventors:

David Yavorsky - Bolton MA, US
John Amara - Reading MA, US
Joaquin Umana - Stoneham MA, US
William Cataldo - Bradford MA, US
Mikhail Kozlov - Lexington MA, US
Matthew Stone - Cambridge MA, US
Assignee:

MILLIPORE CORPORATION - Billerica MA
International Classification:

B01D 15/08
B01D 15/10
B01D 15/36
C07K 1/20
B01D 15/32
C07K 1/22
C07K 1/18
B01D 15/04
B01D 15/38
US Classification:

530413, 2105021, 2101982, 210656, 530416, 530417
Abstract:

Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

Purification Of Immunogens Using A Non-Polysaccharide Matrix
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US Patent:

20120156757, Jun 21, 2012
Filed:

Dec 13, 2011
Appl. No.:

13/324464
Inventors:

Ganapathysubramanian Iyer - Woburn MA, US
Joaquin A. Umana - Stoneham MA, US
Neil Soice - Amherst NH, US
Assignee:

Millipore Corporation - Billerica MA
International Classification:

C12N 7/02
C07K 1/18
C07K 14/155
C07K 14/11
C07K 14/035
C07K 14/005
B01D 15/36
US Classification:

435239, 530413, 521 27
Abstract:

The present invention relates, at least in part, to novel and improved chromatography matrices for separating or purifying immunogens, such as, for example, viruses and viral surface proteins, from one or more contaminants in a sample, where the matrix comprises a porous non-polysaccharide solid support comprising a negatively charged, multivalent ion exchange group directly attached to the solid support.