John William Groves

US Patent:

6699719, Mar 2, 2004

Filed:

Jul 22, 2002

Appl. No.:

10/200682

Inventors:

Miki (Vikky) Yamazaki - Redwood Shores CA
Morrison Ulman - Mountain View CA
Luat T. Nguyen - Fremont CA
Steven G. Boxer - Stanford CA
John T. Groves - Berkeley CA

Assignee:

Proteomic Systems, Inc. - Sunnyvale CA

International Classification:

G01N 2712

US Classification:

436 71, 436164, 436172, 422 8202, 422 8206, 427 213

Abstract:

A surface detector array device suitable for use with a biosensor is disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by a film of aqueous, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Multiplexed assays using the surface detector array device of the present invention are disclosed, as are automated methods for making the surface detector array device that enable formation of arrays wherein the composition of the individual, addressable bilayer regions is unrestricted.

US Patent:

7407768, Aug 5, 2008

Filed:

Sep 11, 2003

Appl. No.:

10/661790

Inventors:

Victoria Yamazaki - San Francisco CA, US
Robert J. Schafer - Sacramento CA, US
Morrison Ulman - Mountain View CA, US
John T. Groves - Berkeley CA, US

Assignee:

Synamem Corporation - Burlingame CA

International Classification:

G01N 33/53

US Classification:

435 72, 4352872, 436517

Abstract:

Membrane-based assays using surface detector array devices suitable for use with a biosensor are disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by an aqueous film, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Arrays may be engineered to display natural membrane materials in a native fluid bilayer configuration, permitting high-throughput discovery of drugs that target and affect membrane components. The membrane-based assays detect binding events by monitoring binding-induced changes in one or more physical properties of fluid bilayers.

US Patent:

8114602, Feb 14, 2012

Filed:

Dec 6, 2004

Appl. No.:

10/581371

Inventors:

John T. Groves - Berkeley CA, US
Michael M. Baksh - Fremont CA, US
Michal Jaros - Brno, CH

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 33/53
C12Q 1/70
B01F 3/12

US Classification:

435 71, 516 77, 435 5

Abstract:

A method and assay are described for measuring the interaction between a ligand and an analyte. The assay can include a suspension of colloidal particles that are associated with a ligand of interest. The colloidal particles are maintained in the suspension at or near a phase transition state from a condensed phase to a dispersed phase. An analyte to be tested is then added to the suspension. If the analyte binds to the ligand, a phase change occurs to indicate that the binding was successful.

US Patent:

20020160505, Oct 31, 2002

Filed:

Feb 13, 2002

Appl. No.:

10/076727

Inventors:

John Groves - Berkeley CA, US
Lara Mahal - New York City NY, US
Carolyn Bertozzi - Berkeley CA, US

International Classification:

C12Q001/00
C12M001/34

US Classification:

435/287100, 435/004000

Abstract:

A method and device for controlled cell adhesion is provided. The device comprises lipid bilayer membranes arranged into discrete areas in a micro-array. They are useful for screening and modulation of living cell adhesion and growth on a solid substrate. The lipid bilayer membranes are doped with various lipids and/or proteins to modulate the adherence of the cells being used in the device.

US Patent:

20080176759, Jul 24, 2008

Filed:

Dec 31, 2007

Appl. No.:

11/968078

Inventors:

Victoria Yamazaki - San Francisco CA, US
Robert J. Schafer - Sacramento CA, US
Morrison Ulman - Mountain View CA, US
John T. Groves - Berkeley CA, US

Assignee:

Synamem Corporation - Burlingame CA

International Classification:

C40B 30/04

US Classification:

506 9

Abstract:

Membrane-based assays using surface detector array devices suitable for use with a biosensor are disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by an aqueous film, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Arrays may be engineered to display natural membrane materials in a native fluid bilayer configuration, permitting high-throughput discovery of drugs that target and affect membrane components. The membrane-based assays detect binding events by monitoring binding-induced changes in one or more physical properties of fluid bilayers.

US Patent:

20080248492, Oct 9, 2008

Filed:

Dec 31, 2007

Appl. No.:

11/968111

Inventors:

Victoria Yamazaki - San Francisco CA, US
Robert J. Schafer - Sacramento CA, US
Morrison Ulman - Mountain View CA, US
John T. Groves - Berkeley CA, US

Assignee:

SYNAMEM CORPORATION, a California Corporation - Burlingame CA

International Classification:

G01N 33/566
G01N 33/68

US Classification:

435 72, 436501

Abstract:

Membrane-based assays using surface detector array devices suitable for use with a biosensor are disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by an aqueous film, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Arrays may be engineered to display natural membrane materials in a native fluid bilayer configuration, permitting high-throughput discovery of drugs that target and affect membrane components. The membrane-based assays detect binding events by monitoring binding-induced changes in one or more physical properties of fluid bilayers.

US Patent:

20080268450, Oct 30, 2008

Filed:

Mar 12, 2008

Appl. No.:

12/047214

Inventors:

Jwa-Min Nam - Seoul, KR
John T. Groves - Berkeley CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12Q 1/68
G01N 33/566

US Classification:

435 6, 436501

Abstract:

The present invention provides a method for detecting an analyte of interest via a bio-barcode assay. The present invention provides a calorimetric bio-barcode method that is capable of detecting minute concentrations of an analyte by relying on porous particles, which enable loading of a large number of barcode DNA per particle, and a metal particle-based colorimetric barcode detection method.

US Patent:

20090011428, Jan 8, 2009

Filed:

Jan 18, 2007

Appl. No.:

12/161553

Inventors:

Jwa-Min Nam - Seoul, KR
Pradeep M. Nair - Berkeley CA, US
Richard M. Neve - San Mateo CA, US
Joe W. Gray - San Francisco CA, US
John T. Groves - Berkeley CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 33/53

US Classification:

435 71

Abstract:

A supported membrane based, strategy for the presentation of soluble signaling molecules to living cells is described. In this system, the fluidity of the supported membrane enables localized enrichment of ligand density in a configuration reflecting cognate receptor distribution on the cell surface. Display of a ligand in non-fluid supported membranes produces significantly less cell adhesion and spreading, thus demonstrating that this technique provides a means to control functional soluble ligand exposure in a surface array format. Furthermore, this technique can be applied to tether natively membrane-bound signaling molecules such as ephrin A1 to a supported lipid bilayer. Such a surface can modulate the spreading behavior of metastatic human breast cancer cells displaying ligands and biomolecules of choice. The SLB microenvironment provides a versatile platform that can be tailored to controllably and functionally present a multitude of cell signaling events in a parallel surface array format.

Author:

John R. Groves

ISBN #:

0897070836

Author:

John Randall Groves

ISBN #:

0827354541

Author:

John Randall Groves

ISBN #:

0827354568

Author:

John Randall Groves

ISBN #:

0766805182

Author:

John Groves

ISBN #:

0407001433

Author:

John Groves

ISBN #:

0407005102

Author:

John Groves

ISBN #:

0713726946