Eric S. Nordman - Palo Alto CA John Shigeura - Fremont CA Albert L. Carrillo - Redwood City CA David M. Demorest - Soquel CA Philip J. Wunderle - El Sobrante CA
A multi-channel capillary electrophoresis apparatus is disclosed. The apparatus includes a capillary array assembly comprising a plurality of capillaries, each capillary having a capillary outlet, and an outlet support for supporting the capillary outlets. The apparatus further includes a cuvette defining a receiving slot, a gap region, and a detection zone, where the receiving slot is adapted to removably receive the outlet support, and wherein when the outlet support is inserted into the receiving slot, the capillary outlets are positioned in the gap region in proximity to the detection zone, and a flow channel is formed by the outlet support and the receiving slot such that the flow channel is in fluid communication with the gap region. In addition, the apparatus includes a front plumbing block in fluid communication with the flow channel for supplying a fluid flow through the gap region sufficient to transport material downstream from the capillary outlets to the detection zone.
Non-Contact Radiant Heating And Temperature Sensing Device For A Chemical Reaction Chamber
An apparatus and methods are provided for heating and sensing the temperature of a chemical reaction chamber without direct physical contact between a heating device and the reaction chamber, or between a temperature sensor and the reaction chamber. A plurality of chemical reaction chambers can simultaneously or sequentially be heated independently and monitored separately.
Time-Delay Integration In Electrophoretic Detection Systems
Eric S. Nordman - Palo Alto CA, US Richard T. Reel - Hayward CA, US John Shigeura - Portola Valley CA, US
Assignee:
Applera Corporation - Foster City CA
International Classification:
G01N021/00
US Classification:
356344
Abstract:
An apparatus for detecting analytes in a sample is provided. The apparatus includes: one or more channels having a detection zone; one or more irradiation sources disposed for irradiating the detection zone with non-coherent radiation; a detector array disposed for collecting light signals emitted from markers in the detection zone excited by the radiation, the detector array having an output; and a system coupled to the detector array for effecting time delay integration of the charges on the detector array corresponding to the light signals by accumulating the charges before reading the charges at the output of the detector array. Other apparatus and methods for detecting analytes in a sample are also provided.
Micro-Channel Design Features That Facilitate Centripetal Fluid Transfer
Sean M. Desmond - San Carlos CA, US John Shigeura - Portola Valley CA, US
Assignee:
Applera Corporation - Foster City CA
International Classification:
G01N 9/30 B01L 11/00 C12M 1/10
US Classification:
422100, 422101, 422102, 436 45, 436177
Abstract:
Microfluidic devices, assemblies, and systems are provided, as are methods of manipulating micro-sized samples of fluids. Microfluidic devices having a plurality of specialized processing features that facilitate centripetal fluid transfer are also provided.
Sample Handling System For A Multi-Channel Capillary Electrophoresis Device
Howard Gregg King - Berkeley CA, US John Shigeura - Portola Valley CA, US Eric S. Nordman - Palo Alto CA, US Sean Matthew Desmond - San Carlos CA, US
Assignee:
Applera Corporation - Foster City CA
International Classification:
G01N 27/447
US Classification:
204601, 204451, 204604, 204453
Abstract:
A sample handling system in a multi-channel capillary electrophoresis apparatus is disclosed. The sample handling system includes a work surface for supporting a plurality of samples located at a plurality of work surface coordinates and a sample loading assembly comprising a plurality of loading wells. At least one of the loading wells includes a capillary fixedly positioned therein. The system further includes a programmable sample transfer device for automatically transferring a sample from a work surface coordinate to a loading well. The invention further includes methods for using the sample handling system.
Analytical Device With Lightguide Illumination Of Capillary And Microgrooves Arrays
Larry J. Carson - Maplewood MN, US Joel R. Dufresne - St. Paul MN, US Patrick R. Fleming - Lake Elmo MN, US Michael C. Lea - Bracknell, GB Nicholas A. Lee - Woodbury MN, US John Shigeura - Portola Valley CA, US
Assignee:
3M Innovative Properties Company - St. Paul MN Applera Corporation - Forster City CA
An analytical cell including a lightguide with a plurality of conduits filled with a migration medium. The medium, the lightguide and a surrounding medium have refractive indices selected such that light entering the lightguide is internally reflected within the lightguide to provide substantially uniform illumination of the conduits.
Zbigniew T. Bryning - Campbell CA, US John Shigeura - Portola Valley CA, US
Assignee:
Applera Corporation - Foster City CA
International Classification:
B01L 3/02
US Classification:
422100, 422 99, 422102, 422103
Abstract:
Microfluidic assemblies, systems, and methods are provided for manipulating fluid samples. Assemblies include an elastically deformable cover layer and a less elastically deformable substrate. The methods include deforming the substrate through the cover layer so that when the cover layer rebounds a new communication results in the assembly between the cover layer and the substrate and/or so that a new barrier wall is formed. Systems for carrying out the methods are also provided.
Microfluidic Size-Exclusion Devices, Systems, And Methods
Sean M. Desmond - San Carlos CA, US Zbigniew T. Bryning - Campbell CA, US John Shigeura - Portola Valley CA, US Gary Lim - San Francisco CA, US Adrian Fawcett - Pleasanton CA, US Jacob K. Freudenthal - San Jose CA, US Gary Bordenkircher - Livermore CA, US
Microfluidic devices, assemblies, and systems are provided, as are methods of manipulating micro-sized samples of fluids. Microfluidic devices having a plurality of specialized processing features are also provided.