- Stanford CA, US Jun Woo Kim - Fullerton CA, US Frank V. Cochran - Stanford CA, US
International Classification:
C07K 14/47 A61K 47/48 C07K 14/81
Abstract:
Disclosed are knottin peptides containing non-natural amino acids so that they can be formed by chemical conjugation into two or more knottin monomers. The knottin monomers comprise a non-natural amino acid such as an aminooxy residue within the polypeptide sequence. The exemplified dimers were produced by oxime formation between two aldehyde groups present on a polyether linker and an aminooxy functional group that was site-specifically incorporated the knottin. Knottins variants based on EETI (trypsin inhibitor) and AgRP (Agouti-related protein) were engineered to contain integrin-binding loops. These dimers were shown to have increased binding strength to integrins on U87MG tumor cells, achieving significant increases in inhibition of cell adhesion and proliferation. Also disclosed are knottin monomers comprising an aminooxy residue; these may be conjugated to molecules such as doxorubicin.
FREESCALE SEMICONDUCTOR Chandler, AZ 2013 to Feb 2015 Photo Equipment TechnicianFAIRCHILD SEMICONDUCTOR Salt Lake City, UT 2010 to 2013 Photo Equipment Support TechnicianINTEL CORPORATION Chandler, AZ 2006 to 2009 Manufacturing Equipment TechnicianHYNIX SEMICONDUCTOR AMERICA Eugene, OR 1998 to 2006 Equipment Engineering TechnicianTWINSTAR SEMICONDUCTOR INC Richardson, TX 1997 to 1998 Equipment Engineering TechnicianMICROCHIP TECHNOLOGY INC Chandler, AZ 1995 to 1997 ERS Technician, ERT memberSYMBIOS LOGIC INC Colorado Springs, CO 1993 to 1995 Equipment Maintenance Technician, TEP memberNATIONAL SEMICONDUCTOR CORP Santa Clara, CA 1990 to 1992 Line Maintenance Technician
Education:
DeVry Institute of Technology Phoenix, AZ Oct 1995 to May 1997 BS in Technical ManagementMission College Santa Clara, CA Sep 1988 to Jan 1991 Certificate in TechnologyKorea University Seoul, KR Mar 1983 to Mar 1984 General Education
Dr. Kim graduated from the University of Louisville School of Medicine in 1998. He works in Louisville, KY and specializes in Family Medicine and Internal Medicine.
Dr. Kim graduated from the University of Maryland School of Medicine in 1994. He works in Culpeper, VA and specializes in Family Medicine. Dr. Kim is affiliated with University Of Virginia Medical Center and UVA Culpeper Hospital.