Karthe Ponnuraj

US Patent:

7850974, Dec 14, 2010

Filed:

Oct 8, 2009

Appl. No.:

12/575909

Inventors:

Magnus Hook - Houston TX, US
Yi Xu - Houston TX, US
Jouko V. Sillanpaa - Houston TX, US
Narayana Sthanam - Birmingham AL, US
Karthe Ponnuraj - Birmingham AL, US
Joseph M. Patti - Cumming GA, US
Jeff T. Hutchins - Cumming GA, US
Andrea Hall - Acworth GA, US
Maria G. Bowden - Sugarland TX, US

Assignee:

The Texas A&M University System - College Station TX
Inhibitex, Inc. - Alpharetta GA
The UAB Research Foundation - Birmingham AL

International Classification:

A61K 39/02

US Classification:

4241901, 4241851, 4242341

Abstract:

A bioinformatic method for identifying and isolating proteins and peptides with MSCRAMM-like characteristics from Gram positive bacteria, such as and bacteria, and proteins and peptides obtained thereby are provided which can be utilized in methods to prevent and treat infections caused by Gram-positive bacteria. The method involves identifying from sequence information those proteins with a putative C-terminal LPXTG (SEQ ID NO:1) cell wall sorting signal and other structural similarities to MSCRAMM proteins having the LPXTG-anchored cell wall proteins. The MSCRAMM proteins and immunogenic regions therein that are identified and isolated using the present invention may be useful in the diagnosis, treatment or prevention of Gram positive bacterial infections.

US Patent:

20040101919, May 27, 2004

Filed:

Sep 15, 2003

Appl. No.:

10/661809

Inventors:

Magnus Hook - Houston TX, US
Yi Xu - Houston TX, US
Jouko Sillanpaa - Houston TX, US
Narayana Sthanam - Birmingham AL, US
Karthe Ponnuraj - Birmingham AL, US
Joseph Patti - Cumming GA, US
Jeff Hutchins - Cumming GA, US
Andrea Hall - Acworth GA, US
Maria Bowden - Sugarland TX, US

International Classification:

G01N033/554
G01N033/569

US Classification:

435/007320

Abstract:

A bioinformatic method is provided for identifying and isolating proteins with MSCRAMM-like characteristics from Gram positive bacteria, such as Enterococcus, Staphylococcus, Streptococcus and Bacillus bacteria, which can then be utilized in methods to prevent and treat infections caused by Gram-positive bacteria. The method involves identifying from sequence information those proteins with a putative C-terminal LPXTG (SEQ ID NO:1) cell wall sorting signal and other structural similarities to MSCRAMM proteins having the LPXTG-anchored cell wall proteins. The MSCRAMM proteins and immunogenic regions therein that are identified and isolated using the present invention may be used to generate antibodies useful in the diagnosis, treatment or prevention of Gram positive bacterial infections.