Kyung M Lee

Address:

10332 Layton Hall Dr APT 409, Fairfax, VA 22030

Phone:

7033524974

VIN:

4T1BK36B17U206777

Make:

TOYOTA

Model:

AVALON

Year:

2007

US Patent:

20110076693, Mar 31, 2011

Filed:

May 15, 2009

Appl. No.:

12/992887

Inventors:

Kyung S. Lee - Gaithersburg MD, US
Jung Eun Park - Bethesda MD, US

Assignee:

The U.S.A. as Represented by the Secretary , Dept.of Health and Human Services (the Gov.) - Bethesda MD

International Classification:

G01N 33/573
C07K 14/00
C07K 7/06
C07K 7/08
C12Q 1/48
G01N 33/53

US Classification:

435 74, 530325, 530350, 530328, 530326, 530324, 530327, 536 231, 435 15, 435 71

Abstract:

Isolated peptide substrates of Plk1 and nucleic acids encoding these peptides are disclosed. The peptides include two to ten repeats of the amino acid sequence set forth as XXAXXXPLHSTXXXXXXX(SEQ ID NO: 1), in which within each repeat X, X, X, X, X, X, X, X, and Xare each independently any amino acid or no amino acid, and Xand Xare each independently any amino acid. Methods of using these peptides to detect Plk1 activity in a sample are also disclosed. In some examples, the method includes contacting a sample with a disclosed peptide substrate of Plk1 in the presence of adenosine triphosphate, or an analog thereof, for a period of time sufficient for Plk1 to phosphorylate the PBIPtide. The presence and/or amount of the phosphorylated and/or the unphosphorylated peptide is detected, thereby detecting and/or quantitating Plk1 kinase activity in the sample.