Ling Yi Wang

US Patent:

6379897, Apr 30, 2002

Filed:

Nov 9, 2000

Appl. No.:

09/710200

Inventors:

Elaine M. Weidenhammer - San diego CA
Ling Wang - San diego CA
Xiao Xu - San diego CA
Michael J. Heller - Encinitas CA
Brenda F. Kahl - San Diego CA

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

C12Q 168

US Classification:

435 6, 435 71, 435 911, 435 912, 4352852, 4352872, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

Abstract:

The present invention presents methods for gene expression monitoring that utilize microelectronic arrays to drive the transport and hybridization of nucleic acids. Procedures are described for generating mRNA expression samples for use in these methods from populations of cells, tissues, or other biological source materials, that may differ in their physiological and/or pathological state. Provided in the invention are methods for generating a reusable nucleic acid transcript library from mRNA in a sample of biological material. In order to improve gene expression monitoring on the microelectronic arrays, the transcripts are amplified to produce sample nucleic acid amplicons of a defined length. Because multiple sample amplicons may be selectively hybridized to controlled sites in the electronic array, the gene expression profiles of the polynucleotide populations from different sources can be directly compared in an array format using electronic hybridization methodologies. Also provided in the invention are methods for detecting the level of sample amplicons using electronically assisted primer extension detection, and utilizing individual test site hybridization controls.

US Patent:

6468742, Oct 22, 2002

Filed:

Apr 12, 1999

Appl. No.:

09/291129

Inventors:

Michael I. Nerenberg - La Jolla CA
David M. Canter - San Diego CA
Ray R. Radtkey - San Diego CA
Ling Wang - San Diego CA
James P. Oconnell - Solana Beach CA
Ronald G. Sosnowski - Coronado CA

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

C12Q 168

US Classification:

435 6, 422 50, 422 681

Abstract:

Methods are provided for the analysis and determination of the nature of single nucleic acid polymorphisms (SNPs) in a genetic target. In one method of this invention, the nature of the SNPs in the genetic target is determined by the steps of providing a plurality of hybridization complexes arrayed on a plurality of test sites on an electronically bioactive microchip, where the hybridization complex includes at least a nucleic acid target containing a SNP, a stabilizer probe having a sequence complementary to the target sequence and/or reporter probe, and a reporter probe having a selected sequence complementary to either the stabilizer or the same target sequence strand wherein a selected sequence of the reporter includes either a wild type nucleotide or a nucleotide corresponding to the SNP of the target. In accordance with the invention, the stabilizer, reporter and target amplicons are hybridized using electronic assistance of the microchip system such that base-stacking energies are utilized in discerning among other identifying indicators, the presence of wild type or polymorphism sequence. Applications include disease diagnostics, such as for the identification of polymorphisms in structural genes, regulatory regions, antibiotic or chemotherapeutic resistance conferring regions, or for SNPs associated with speciation or used for determination of genetic linkage.

US Patent:

6492122, Dec 10, 2002

Filed:

Oct 10, 2001

Appl. No.:

09/975408

Inventors:

Elaine M. Weidenhammer - San Diego CA
Ling Wang - San Diego CA
Xiao Xu - San Diego CA
Michael J. Heller - Encinitas CA
Brenda F. Kahl - San Diego CA

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

C12Q 168

US Classification:

435 6, 435 71, 435 911, 435 912, 4352872, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

Abstract:

The present invention presents techniques useful in methods for gene expression monitoring, and other nucleic acid hybridization assays, that utilize microelectronic arrays to drive the transport and hybridization of nucleic acids. Particularly, methods for normalizing the signals of individual microlocations by the use of an internal control sequence probe are provided. These methods are particularly useful for hybridization assays in which a quantitative comparison of the hybridization of several different sequences at a plurality of microlocations is desired, such as in gene expression analyses.

US Patent:

7582421, Sep 1, 2009

Filed:

Sep 16, 2002

Appl. No.:

10/245206

Inventors:

Ronald G. Sosnowski - Coronado CA, US
Michael I. Nerenberg - La Jolla CA, US
David M. Canter - San Diego CA, US
Ray R. Radtkey - San Diego CA, US
Ling Wang - San Diego CA, US
James P. O'Connell - Solana Beach CA, US

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04
C07H 21/00

US Classification:

435 6, 435 911, 435 912, 536 231, 536 243, 536 2433, 536 253, 536 2532

Abstract:

This application includes methods for detecting single nucleotide polymorphisms (SNPs) in a sample using an electronically addressable microchip having a plurality of test sites. A sample nucleic acid is electronically biased, concentrated at, and immobilized to a test site on the microchip. A mixture comprising a first labeled probe and a second labeled probe is electronically hybridized to the sample nucleic acid to form first or second hybridized complexes. The first labeled probe is perfectly complementary to the first sample nucleic acid and the second labeled probe is complementary to the sample nucleic acid and contains a nucleotide that forms a mismatch with the nucleotide at the site of the polymorphism. The first or second hybridized complexes are detected by determining a signal intensity of the label of the first or second probe.

US Patent:

7910267, Mar 22, 2011

Filed:

Dec 12, 2008

Appl. No.:

12/333699

Inventors:

Xianzhong Zeng - Fremont CA, US
Hai Sun - Milpitas CA, US
Hongping Yuan - Fremont CA, US
Dujiang Wan - Fremont CA, US
Ling Wang - Hercules CA, US

Assignee:

Western Digital (Fremont), LLC - Fremont CA

International Classification:

G03F 1/00

US Classification:

430 5, 430320

Abstract:

An optical mask for providing a pattern for portion of an electronic device, such as a magnetic recording transducer, is disclosed. The optical mask includes a device feature and at least one detached correction feature. The device feature includes a corner corresponding to a device corner of the pattern. The device corner has an angle of greater than zero degrees and less than one hundred eighty degrees. The at least one detached correction feature resides in proximity to but is physically separated from the corner. Each of the at least one detached correction feature is sub-resolution.

US Patent:

8322023, Dec 4, 2012

Filed:

Nov 10, 2009

Appl. No.:

12/615544

Inventors:

Xianzhong Zeng - Fremont CA, US
Yunhe Huang - Pleasanton CA, US
Ling Wang - Hercules CA, US
Hai Sun - Milpitas CA, US

Assignee:

Western Digital (Fremont), LLC - Fremont CA

International Classification:

G11B 5/127
H04R 31/00

US Classification:

2960316, 28960312, 28960314, 28960315, 28960318, 216 62, 216 65, 216 66, 360121, 360122, 360317, 451 5, 451 41

Abstract:

A method for fabricating a magnetic transducer is described. The magnetic transducer includes a pole having a pole tip and a flared region. The method Includes providing a first mask layer on the pole and providing a second mask layer on the first mask layer. The first mask layer is soluble in a predetermined solution and has a first thickness. The second mask layer has a second thickness greater than the first thickness. The method also includes forming a mask from the first mask layer and the second mask layer. The step of forming the mask layer includes using the predetermined solution. The mask has a pattern that exposes a portion of the pole tip and covers a portion of the flared region. The method also includes providing a wrap-around shield on at least the pole tip.

US Patent:

8394280, Mar 12, 2013

Filed:

Nov 6, 2009

Appl. No.:

12/614210

Inventors:

Dujiang Wan - Fremont CA, US
Hai Sun - Milpitas CA, US
Hongping Yuan - Fremont CA, US
Ling Wang - Hercules CA, US
Xianzhong Zeng - Fremont CA, US

Assignee:

Western Digital (Fremont), LLC - Fremont CA

International Classification:

B44C 1/22

US Classification:

216 22, 216 41, 216 47, 216 57, 216 67, 216 72, 216 83, 216 95, 438694, 438703, 438736, 438737, 438738

Abstract:

Methods of patterning a material are disclosed. A first resist pattern is formed on a field. A protective layer is formed over the first resist pattern and at least a portion of the field. A second resist pattern is formed over a portion of the protective layer. A portion of a material to be patterned deposited adjacent to the first and second resist patterns is removed.

US Patent:

8618110, Dec 31, 2013

Filed:

Feb 9, 2010

Appl. No.:

12/702651

Inventors:

Jay Jie-Qiang Wu - Fremont CA, US
Ling Wang - Union City CA, US

Assignee:

VM Discovery Inc. - Fremont CA

International Classification:

A61K 31/5377
A61K 31/496
A61K 31/454
A61K 31/422
A61K 31/4192
C07D 487/04
C07D 417/14
C07D 413/14
C07D 413/12
C07D 413/10
C07D 405/14
C07D 401/14
C07D 401/06
C07D 403/14
C07D 403/10
C07D 403/06

US Classification:

51425406, 51425218, 51425219, 51425304, 5142531, 51425311, 51425402, 51425404, 5142328, 514278, 514322, 514375, 514377, 514359, 544121, 544349, 544357, 544364, 544367, 544366, 548217, 548259, 546 16, 546199

Abstract:

The present invention includes and relates generally to compounds of structural Formula (I), or a salt, solvate, or prodrug thereof, which modulate apoptosis in cells. The present invention also provides pharmaceutical compositions containing these compounds, methods of making these compounds, and methods of using these compounds and pharmaceutical compositions for treatment of diseases associated with irregular apoptosis in cells.

Author:

Ling Wang

ISBN #:

0141003170

Author:

Ling Wang

ISBN #:

0521072352

Author:

Ling Wang

ISBN #:

0521322766

Author:

Ling Wang

ISBN #:

0521652707