Ling C Wang

US Patent:

6379897, Apr 30, 2002

Filed:

Nov 9, 2000

Appl. No.:

09/710200

Inventors:

Elaine M. Weidenhammer - San diego CA
Ling Wang - San diego CA
Xiao Xu - San diego CA
Michael J. Heller - Encinitas CA
Brenda F. Kahl - San Diego CA

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

C12Q 168

US Classification:

435 6, 435 71, 435 911, 435 912, 4352852, 4352872, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

Abstract:

The present invention presents methods for gene expression monitoring that utilize microelectronic arrays to drive the transport and hybridization of nucleic acids. Procedures are described for generating mRNA expression samples for use in these methods from populations of cells, tissues, or other biological source materials, that may differ in their physiological and/or pathological state. Provided in the invention are methods for generating a reusable nucleic acid transcript library from mRNA in a sample of biological material. In order to improve gene expression monitoring on the microelectronic arrays, the transcripts are amplified to produce sample nucleic acid amplicons of a defined length. Because multiple sample amplicons may be selectively hybridized to controlled sites in the electronic array, the gene expression profiles of the polynucleotide populations from different sources can be directly compared in an array format using electronic hybridization methodologies. Also provided in the invention are methods for detecting the level of sample amplicons using electronically assisted primer extension detection, and utilizing individual test site hybridization controls.

US Patent:

6468742, Oct 22, 2002

Filed:

Apr 12, 1999

Appl. No.:

09/291129

Inventors:

Michael I. Nerenberg - La Jolla CA
David M. Canter - San Diego CA
Ray R. Radtkey - San Diego CA
Ling Wang - San Diego CA
James P. Oconnell - Solana Beach CA
Ronald G. Sosnowski - Coronado CA

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

C12Q 168

US Classification:

435 6, 422 50, 422 681

Abstract:

Methods are provided for the analysis and determination of the nature of single nucleic acid polymorphisms (SNPs) in a genetic target. In one method of this invention, the nature of the SNPs in the genetic target is determined by the steps of providing a plurality of hybridization complexes arrayed on a plurality of test sites on an electronically bioactive microchip, where the hybridization complex includes at least a nucleic acid target containing a SNP, a stabilizer probe having a sequence complementary to the target sequence and/or reporter probe, and a reporter probe having a selected sequence complementary to either the stabilizer or the same target sequence strand wherein a selected sequence of the reporter includes either a wild type nucleotide or a nucleotide corresponding to the SNP of the target. In accordance with the invention, the stabilizer, reporter and target amplicons are hybridized using electronic assistance of the microchip system such that base-stacking energies are utilized in discerning among other identifying indicators, the presence of wild type or polymorphism sequence. Applications include disease diagnostics, such as for the identification of polymorphisms in structural genes, regulatory regions, antibiotic or chemotherapeutic resistance conferring regions, or for SNPs associated with speciation or used for determination of genetic linkage.

US Patent:

6492122, Dec 10, 2002

Filed:

Oct 10, 2001

Appl. No.:

09/975408

Inventors:

Elaine M. Weidenhammer - San Diego CA
Ling Wang - San Diego CA
Xiao Xu - San Diego CA
Michael J. Heller - Encinitas CA
Brenda F. Kahl - San Diego CA

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

C12Q 168

US Classification:

435 6, 435 71, 435 911, 435 912, 4352872, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

Abstract:

The present invention presents techniques useful in methods for gene expression monitoring, and other nucleic acid hybridization assays, that utilize microelectronic arrays to drive the transport and hybridization of nucleic acids. Particularly, methods for normalizing the signals of individual microlocations by the use of an internal control sequence probe are provided. These methods are particularly useful for hybridization assays in which a quantitative comparison of the hybridization of several different sequences at a plurality of microlocations is desired, such as in gene expression analyses.

US Patent:

6636005, Oct 21, 2003

Filed:

Nov 14, 2001

Appl. No.:

09/991082

Inventors:

Ihor Terence Wacyk - Briarcliff Manor NY
Ling Wang - Millwood NY
Johannes Hendrik Wessels - Mierlo, NL

Assignee:

Koninklijke Philips Eletronics N.V. - Eindhoven

International Classification:

G05F 100

US Classification:

315291, 315DIG 4, 315149, 315294

Abstract:

The invention is a new architecture for a high frequency (HF) ballast with wireless communication interface. The new architecture integrates the RF wireless interface into the ballast. A user control transmits an RF control signal to a second antenna at the ballast site which provides the RF signal to the ballast which activates the fluorescent lamp. The ballast includes a transceiver/receiver, a communication decoder, a power control stage and a power stage. The transceiver/receiver receives the RF signal and communicates it to the communication decoder which acts as an interface to the power stage control. The power stage control controls the power stage that activates the fluorescent lamp. The communication decoder, power control stage, power stage and transceiver/receiver are located within the ballast enclosure which is an important part of the invention. If the power stage control is digital it may be combined with the communication decoder into one microprocessor or digital controller such as an ASIC.

US Patent:

6831569, Dec 14, 2004

Filed:

Mar 8, 2001

Appl. No.:

09/802104

Inventors:

Ling Wang - Ossining NY
Ihor Wacyk - Briarcliff Manor NY

Assignee:

Koninklijke Philips Electronics N.V. - Eindhoven

International Classification:

G08C 1900

US Classification:

34082552, 340 35

Abstract:

A system for implementing a method for initializing and binding ballasts is disclosed. The system comprises a remote control having a master controller and the ballasts each having a slave controller. The master controller and the slave controllers are operated to implement routines whereby the master controller generates a clock sequence of a plurality of clock cycles, and the slave controllers randomly generate addresses and direct a transmission of signals indicative of the generated random addresses to the master controller during corresponding clock cycles. In response thereto, the master controller assigns network addresses corresponding to the random addresses as indicated by the corresponding clock cycles. The master controller and the slave controllers are further operated to implement routines for verifying the assigned network addresses and routines for binding each network address to a command of the remote control.

US Patent:

6859644, Feb 22, 2005

Filed:

Dec 19, 2002

Appl. No.:

10/323414

Inventors:

Ling Wang - Millwood NY, US

Assignee:

Koninklijke Philips Electronics N.V. - Eindhoven

International Classification:

H04B001/18
G05B019/18
H04L012/28
H05B037/02

US Classification:

4551592, 4551512, 700 3, 700 11, 370908, 370910, 315153, 315316, 315312

Abstract:

A method of initializing system components of a wireless-controlled lighting system. The system components include a remote control and a plurality of lighting units which communicate with a control master for the system via commonly-received radio communications. In order to become part of the system, each component transmits a respective request for initialization. A local control master for the system responds to each request, in turn, by allocating and transmitting a unique ID code for the requesting component. It then transmits a verify command to the requesting component which, if it has received the ID code, signals the user affirmatively.

US Patent:

7554274, Jun 30, 2009

Filed:

Dec 8, 2003

Appl. No.:

10/538605

Inventors:

Ling Wang - Millwood NY, US
Demetri J. Giannopoulos - Norwalk CT, US

Assignee:

Koninklijke Philips Electronics N.V. - Eindhoven

International Classification:

H05B 41/00
H02J 3/14

US Classification:

315317, 315321, 307 40

Abstract:

The present invention provides a master-slave architecture for a radio frequency RF networked lighting control system having all slave elements (ballasts) configured as backups for a network master control unit. In the system and method of the present invention a slave element can become the network master network unit without reconfiguring the network and without any human intervention. Similarly, both a master and one or more slave elements may recover from a temporary outage without necessitating reconfiguration of the network and without any human intervention.

US Patent:

7582421, Sep 1, 2009

Filed:

Sep 16, 2002

Appl. No.:

10/245206

Inventors:

Ronald G. Sosnowski - Coronado CA, US
Michael I. Nerenberg - La Jolla CA, US
David M. Canter - San Diego CA, US
Ray R. Radtkey - San Diego CA, US
Ling Wang - San Diego CA, US
James P. O'Connell - Solana Beach CA, US

Assignee:

Nanogen, Inc. - San Diego CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04
C07H 21/00

US Classification:

435 6, 435 911, 435 912, 536 231, 536 243, 536 2433, 536 253, 536 2532

Abstract:

This application includes methods for detecting single nucleotide polymorphisms (SNPs) in a sample using an electronically addressable microchip having a plurality of test sites. A sample nucleic acid is electronically biased, concentrated at, and immobilized to a test site on the microchip. A mixture comprising a first labeled probe and a second labeled probe is electronically hybridized to the sample nucleic acid to form first or second hybridized complexes. The first labeled probe is perfectly complementary to the first sample nucleic acid and the second labeled probe is complementary to the sample nucleic acid and contains a nucleotide that forms a mismatch with the nucleotide at the site of the polymorphism. The first or second hybridized complexes are detected by determining a signal intensity of the label of the first or second probe.

Author:

Ling Wang

ISBN #:

0141003170

Author:

Ling Wang

ISBN #:

0521072352

Author:

Ling Wang

ISBN #:

0521322766

Author:

Ling Wang

ISBN #:

0521652707