The present invention presents methods for gene expression monitoring that utilize microelectronic arrays to drive the transport and hybridization of nucleic acids. Procedures are described for generating mRNA expression samples for use in these methods from populations of cells, tissues, or other biological source materials, that may differ in their physiological and/or pathological state. Provided in the invention are methods for generating a reusable nucleic acid transcript library from mRNA in a sample of biological material. In order to improve gene expression monitoring on the microelectronic arrays, the transcripts are amplified to produce sample nucleic acid amplicons of a defined length. Because multiple sample amplicons may be selectively hybridized to controlled sites in the electronic array, the gene expression profiles of the polynucleotide populations from different sources can be directly compared in an array format using electronic hybridization methodologies. Also provided in the invention are methods for detecting the level of sample amplicons using electronically assisted primer extension detection, and utilizing individual test site hybridization controls.
Methods For Determination Of Single Nucleic Acid Polymorphisms Using Bioelectronic Microchip
Michael I. Nerenberg - La Jolla CA David M. Canter - San Diego CA Ray R. Radtkey - San Diego CA Ling Wang - San Diego CA James P. Oconnell - Solana Beach CA Ronald G. Sosnowski - Coronado CA
Assignee:
Nanogen, Inc. - San Diego CA
International Classification:
C12Q 168
US Classification:
435 6, 422 50, 422 681
Abstract:
Methods are provided for the analysis and determination of the nature of single nucleic acid polymorphisms (SNPs) in a genetic target. In one method of this invention, the nature of the SNPs in the genetic target is determined by the steps of providing a plurality of hybridization complexes arrayed on a plurality of test sites on an electronically bioactive microchip, where the hybridization complex includes at least a nucleic acid target containing a SNP, a stabilizer probe having a sequence complementary to the target sequence and/or reporter probe, and a reporter probe having a selected sequence complementary to either the stabilizer or the same target sequence strand wherein a selected sequence of the reporter includes either a wild type nucleotide or a nucleotide corresponding to the SNP of the target. In accordance with the invention, the stabilizer, reporter and target amplicons are hybridized using electronic assistance of the microchip system such that base-stacking energies are utilized in discerning among other identifying indicators, the presence of wild type or polymorphism sequence. Applications include disease diagnostics, such as for the identification of polymorphisms in structural genes, regulatory regions, antibiotic or chemotherapeutic resistance conferring regions, or for SNPs associated with speciation or used for determination of genetic linkage.
Quantitative Analysis Methods On Active Electronic Microarrays
The present invention presents techniques useful in methods for gene expression monitoring, and other nucleic acid hybridization assays, that utilize microelectronic arrays to drive the transport and hybridization of nucleic acids. Particularly, methods for normalizing the signals of individual microlocations by the use of an internal control sequence probe are provided. These methods are particularly useful for hybridization assays in which a quantitative comparison of the hybridization of several different sequences at a plurality of microlocations is desired, such as in gene expression analyses.
Digital Phase Locked Loop With Programmable Digital Filter
Kuang Chi - San Jose CA, US Ling Wang - Sunnyvale CA, US Antony Davies - Campbell CA, US
Assignee:
Lattice Semiconductor Corporation - Hillsboro OR
International Classification:
H03D 3/24
US Classification:
375376, 375374, 375375
Abstract:
In a digital filter of a DPLL (digital phase locked loop) for minimizing the bit error rate for multiple communications protocols, a first reloadable register portion stores a TBW (total bandwidth) value programmed into the first reloadable register portion through a first port, and a second reloadable register portion stores a DBW (differential bandwidth) value programmed into the second reloadable register portion through a second port. An upcounter generates an UPCNT value by counting up each UP signal pulse generated by a phase transition detector when a first phase of a SDIN (serial data input) signal leads a second phase of a current ACLK (recovered clock) signal. A downcounter generates a DOWNCNT value by counting up each DOWN signal pulse generated by the phase transition detector when the first phase of the SDIN (serial data input) signal lags the second phase of the current ACLK (recovered clock) signal. One of a FWD (forward) signal or a BWD (backward) signal are asserted or are both not asserted depending on the UPCNT value and the DNCNT value in comparison to the TBW value and the DBW value. Another clock signal having a leading phase from the current ACLK signal is selected as a new ACLK (recovered clock) signal when the FWD signal is asserted.
Digital Phase Locked Loop With Phase Selector Having Minimized Number Of Phase Interpolators
Antony Davies - Campbell CA, US Chienkuang Chen - Sunnyvale CA, US Ling Wang - Sunnyvale CA, US
Assignee:
Lattice Semiconductor Corporation - Hillsboro OR
International Classification:
H03D 3/24
US Classification:
375376, 375374, 375375
Abstract:
In one embodiment of the invention, a phase selection unit for generating a recovered clock signal (SCLK), a phase select signal generator generates a phase select signals in response to a FWD signal and a BWD signal from a digital filter. The digital filter asserts the FWD signal if the phase of a SDIN (serial digital input) signal leads the phase of the recovered clock signal, and asserts the BWD signal if the phase of the SDIN (serial digital input) signal lags the phase of the recovered clock signal. A multiplexer receives a number of given clock signals arranged in a predetermined phase order and outputs selected first and second output clock signals, each being one of the given clock signals. A phase interpolator receives the selected first and second output clock signals from the multiplexer to generate the recovered clock signal having a phase that is phase interpolated between the phases of the first and second output clock signals.
Methods For Determination Of Single Nucleic Acid Polymorphisms Using A Bioelectronic Microchip
Ronald G. Sosnowski - Coronado CA, US Michael I. Nerenberg - La Jolla CA, US David M. Canter - San Diego CA, US Ray R. Radtkey - San Diego CA, US Ling Wang - San Diego CA, US James P. O'Connell - Solana Beach CA, US
This application includes methods for detecting single nucleotide polymorphisms (SNPs) in a sample using an electronically addressable microchip having a plurality of test sites. A sample nucleic acid is electronically biased, concentrated at, and immobilized to a test site on the microchip. A mixture comprising a first labeled probe and a second labeled probe is electronically hybridized to the sample nucleic acid to form first or second hybridized complexes. The first labeled probe is perfectly complementary to the first sample nucleic acid and the second labeled probe is complementary to the sample nucleic acid and contains a nucleotide that forms a mismatch with the nucleotide at the site of the polymorphism. The first or second hybridized complexes are detected by determining a signal intensity of the label of the first or second probe.
Ariel Fuxman - San Jose CA, US Peter Jay Haas - San Jose CA, US Berthold Reinwald - San Jose CA, US Yannis Sismanis - San Jose CA, US Ling Wang - San Jose CA, US
Assignee:
International Business Machines Corporation - Armonk NY
International Classification:
G06F 17/30 G06F 7/00
US Classification:
707769, 707602, 707718
Abstract:
A method is disclosed for conducting a query to transform data in a pre-existing database, the method comprising: collecting database information from the pre-existing database, the database information including inconsistent dimensional tables and fact tables; running an entity discovery process on the inconsistent dimensional tables and the fact tables to produce entity mapping tables; using the entity mapping tables to resolve the inconsistent dimensional tables into resolved dimensional tables; and running the query on a resolved database to obtain a query result, the resolved database including the resolved dimensional table.
Method And System For Providing Optical Proximity Correction For Structures Such As A Pmr Nose
Xianzhong Zeng - Fremont CA, US Hai Sun - Milpitas CA, US Hongping Yuan - Fremont CA, US Dujiang Wan - Fremont CA, US Ling Wang - Hercules CA, US
Assignee:
Western Digital (Fremont), LLC - Fremont CA
International Classification:
G03F 1/00
US Classification:
430 5, 430320
Abstract:
An optical mask for providing a pattern for portion of an electronic device, such as a magnetic recording transducer, is disclosed. The optical mask includes a device feature and at least one detached correction feature. The device feature includes a corner corresponding to a device corner of the pattern. The device corner has an angle of greater than zero degrees and less than one hundred eighty degrees. The at least one detached correction feature resides in proximity to but is physically separated from the corner. Each of the at least one detached correction feature is sub-resolution.
Name / Title
Company / Classification
Phones & Addresses
Ling Wang President
Gentarget Inc. Commercial Physical and Biological Research
9865 Mesa Rim Rd Ste 207, San Diego, CA 92121
Ling Wang President
Gslp Inc Software Developer
4125 Mohr Ave, Pleasanton, CA 94566
Ling Wang President
Hswl, Inc
9608 Las Tunas Dr, Temple City, CA 91780
Ling Wang Owner
Wang's Acupunture Clinic Health Practitioner's Office
1929 Irving St, San Francisco, CA 94122 PO Box 532, Millbrae, CA 94030
Huawei Technologies Inc Santa Clara, CA Jan 2014 to Jul 2014 Fixed Asset Administrator (Contractor)I-Tech Company, Fremont, CA Fremont, CA 2011 to 2013 Account Payable & LogisticsNan Fang Group, Inc. San Leandro, CA San Leandro, CA 2010 to 2011 Account Payable and AdministratorChinese Consumer Yellow Page Union City, CA 2009 to 2010 Account ExecutiveBay Cal Financial Corporation Milpitas, CA 2009 to 2009 Loan ProcessorT. T. International, Fremont, CA Fremont, CA 2005 to 2007 Account Payable (part time),China Travel Services Group Company Hong Kong, Hong Kong Island 1998 to 1999 Bookkeeper
Education:
California State University 2008 Bachelor of Science in Business AdministrationOhlone College Fremont, CA 2002 Certificate
Human Factor International Fairfield, IA Oct 2013 to Aug 2014 Lead Usability SpecialistHuman Factor International
Aug 2012 to Aug 2013 Associate Business Development ManagerUbisoft Entertainment Montral, QC Nov 2011 to Jun 2012 Data Analyst
Education:
School of Politics and Economics Claremont, CA 2009 to 2011 Master of Arts in EconomicsThree Gorges University Yichang, China 2004 to 2008 Bachelor of Arts in EconomicsThree Gorges University Yichang, China 2005 to 2007 Bachelor of Arts in English
Skills:
Excel, SQL Management Studio, Tableau, PowerPoint, Outlook, Word, Stata
Nov 2012 to 2000 Assistant Manager/ Product Knowledge SpecialistSephora Palo Alto, CA Sep 2008 to Aug 2010 Skincare Consultant/Client Service RepresentativeFremont Vista/Hill Retirement Home Fremont, CA Nov 2006 to Aug 2007 Concierge/ Activities Assistant
Education:
Fashion Institute of Design &Merchandising Jun 2013 AA
Skills:
Computer Program include: Microsoft Word, Excel, Adobe illustrator, Photoshop, Salesforce. Extensive knowledge in the beauty and styling industry. Attended SOS Cosmetics indstry traiing in SF via Sephora.
RALINK TECHNOLOGY CORPORATION, A MEDIATEK COMPANY Cupertino, CA 2008 to 2010 Strategic Marketing/Business Development ManagerUBICOM Inc Sunnyvale, CA 2008 to 2008 Senior Product Marketing ManagerVENTURI WIRELESS Sunnyvale, CA 2006 to 2007 Director, Program Management, Product Management GroupPHILIPS ELECTRONICS NORTH AMERICA Briarcliff Manor, NY Dec 1998 to Sep 2006 Acting Product Manager / Senior Member of Technical Staff (2002 ~ 2006); Member of Technical Staff (1998 ~ 2002)
Education:
KELLOGG SCHOOL OF MANAGEMENT, NORTHWESTERN UNIVERSITY Chicago, IL 2006 Master of Business Administration in Management & StrategyRENSSELAER POLYTECHNIC INSTITUTE Troy, NY 1998 Master of Science in Electrical EngineeringTSINGHUA UNIVERSITY 1995 Bachelor of Science in Engineering Physics
Skills:
Strategic marketing, Strategic planning, business development, partnership development, product management
eriodically throughout Chinese history, rulers have sought to centralize control and isolate China from the outside. As Fei-Ling Wang of the Georgia Institute of Technology pointed out in The China Order: Centralia, World Empire, and the Nature of Chinese Power, such attempts result in disaster for China.
Date: Jul 15, 2022
Category: Business
Source: Google
Chinese COVID-19 Vaccine Phase 2 Trial Results: Safe and Induces an Immune Response
unogenicity and safety of a recombinant adenovirus type-5-vectored COVID-19 vaccine in healthy adults aged 18 years or older: a randomised, double-blind, placebo-controlled, phase 2 trial by Feng-Cai Zhu, Xu-Hua Guan, Yu-Hua Li, Jian-Ying Huang, Tao Jiang, Li-Hua Hou, Jing-Xin Li, Bei-Fang Yang, Ling Wang, We