Malford E Cullum from 440 Wicks St, Volo, IL 60030, age 73, Phone: 8475483730

Us Patents

Rapid Immunoassay Of Anthrax Protective Antigen In Vaccine Cultures And Bodily Fluids By Fluorescence Polarization
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US Patent:

7504202, Mar 17, 2009
Filed:

Mar 26, 2004
Appl. No.:

10/809877
Inventors:

Malford E. Cullum - Grayslake IL, US
Paul Hine - Holt MI, US
Lloyd G. Simonson - Spring Grove IL, US
Chun N. Shih - East Lansing MI, US
Diane R. Bienek - Lindenhurst IL, US
Sukjoon Park - Germantown MD, US
Linda A. Lininger - Grayslake IL, US
Assignee:

The United States of America as represented by the Secretary of the Navy - Washington DC
International Classification:

C12Q 1/00
US Classification:

435 4, 435 793, 435 34, 436518, 530350
Abstract:

The inventive subject matter relates to a competitive method for estimating the concentration in a sample of a protein or antibody thereof selected from the group consisting of protective antigen (PA), lethal factor (LF) and edema factor (EF). The method may employ the use of Fluorescence Polarization, FLT or FRET. The competitive methods are capable of detecting a target protein within 5 minutes within the range of 0. 1 to 10. 0 nM. The methods provide for the rapid detection and quantitation of bacteria, bacterial antigen or antibody in culture media or broth of growing cultures of bacteria, including by fluorescent methods.

Hand-Held Fluorescence Polarimeter
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US Patent:

7528952, May 5, 2009
Filed:

Jun 2, 2005
Appl. No.:

11/148572
Inventors:

Malford E. Cullum - Grayslake IL, US
Istvan Naday - Naperville IL, US
Alan L. McArthur - Mokena IL, US
Assignee:

The United States of America as represented by the Secretary of the Navy - Washington DC
International Classification:

G01J 3/00
G01J 4/00
C12M 1/00
C12M 3/00
US Classification:

356368, 356300, 356364, 4352831, 4352871, 4352872
Abstract:

A small, portable, hand-sized apparatus for detecting microorganisms or chemicals in liquid samples by fluorescence polarization. The apparatus operates using a low power excitation light source, such as an LED, in order to irradiate a sample with polarized light. Detection of emitted polarized light from the sample is detected in multiple planes simultaneously using low power detectors resulting in an elimination of error caused by drifts in intensity in sequential measurements and in reducing assay time.

Fluorescence Polarization Instruments And Methods For Detection Of Exposure To Biological Materials By Fluorescence Polarization Immunoassay Of Saliva, Oral Or Bodily Fluids
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US Patent:

8114582, Feb 14, 2012
Filed:

Jun 27, 2008
Appl. No.:

12/163412
Inventors:

Malford E. Cullum - Grayslake IL, US
Lloyd G. Simonson - Spring Grove IL, US
Sylvia Z. Schade - Riverside IL, US
Linda A. Lininger - Grayslake IL, US
Alan L. McArthur - Mokena IL, US
Assignee:

The United States of America as represented by the Secretary of the Navy - Washington DC
International Classification:

C12Q 1/00
G01N 33/53
G01N 33/554
G01N 3/00
A61K 39/00
A61K 39/07
A61K 39/04
US Classification:

435 4, 435 71, 435 732, 435 792, 4241841, 4242461, 4242481
Abstract:

The inventive subject matter relates to a method for detecting the presence of a biological substance of interest in a test sample of saliva or oral fluid, comprising combining said test sample with a fluorescence-labeled ligand to said biological substance and detecting a change in the fluorescence polarization of said test sample produced by binding of said fluorescence-labeled ligand to said biological substance. In one aspect of the inventive subject matter, said method comprises additional steps for comparing the fluorescence polarization of said test sample with the fluorescence polarization of a control solution. Also provided is a miniaturized, portable apparatus for measuring the fluorescence polarization of a liquid sample.

Fluorescence Polarization Instruments And Methods For Detection Of Exposure To Biological Materials By Fluorescence Polarization Immunoassay Of Saliva, Oral Or Bodily Fluids
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US Patent:

20050095601, May 5, 2005
Filed:

Nov 5, 2003
Appl. No.:

10/700868
Inventors:

Malford Cullum - Grayslake IL, US
Lloyd Simonson - Spring Grove IL, US
Sylvia Schade - Riverside IL, US
Linda Lininger - Grayslake IL, US
Alan McArthur - Mokena IL, US
International Classification:

C12Q001/68
G01N033/53
US Classification:

435006000, 435007100
Abstract:

The inventive subject matter relates to a method for detecting the presence of a biological substance of interest in a test sample of saliva or oral fluid, comprising combining said test sample with a fluorescence-labeled ligand to said biological substance and detecting a change in the fluorescence polarization of said test sample produced by binding of said fluorescence-labeled ligand to said biological substance. In one aspect of the inventive subject matter, said method comprises additional steps for comparing the fluorescence polarization of said test sample with the fluorescence polarization of a control solution. Also provided is a miniaturized, portable apparatus for measuring the fluorescence polarization of a liquid sample.

Method For The Rapid Diagnosis Of Infectious Disease By Detection And Quantitation Of Microorganism Induced Cytokines
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US Patent:

20050164168, Jul 28, 2005
Filed:

May 28, 2004
Appl. No.:

10/855325
Inventors:

Malford Cullum - Grayslake IL, US
Gerald Mazurek - Dunwoody GA, US
International Classification:

C12Q001/70
G01N033/53
G01N033/569
G01N033/554
US Classification:

435005000, 435007220, 435007320
Abstract:

The inventive subject matter relates to a competitive method for the diagnosis of latent infectious disease, such as Mycobacterium tuberculosis, by estimating, the concentration of cytokine, such as interferon-gamma produced by stimulated immune cells, collected from whole blood, by Fluorescence Polarization (FP), Fluorescence Resonance Energy Transfer (FRET) or Fluorescence Lifetime (FLT) due to antibody-cytokine interaction or by dimerization of the cytokine.

Method For The Detection Of Stress Biomarkers Including Cortisol By Fluorescence Polarization
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US Patent:

20060105397, May 18, 2006
Filed:

Jan 4, 2006
Appl. No.:

11/328486
Inventors:

Malford Cullum - Grayslake IL, US
Christopher Duplessis - Chesapeake CT, US
Loring Crepeau - Groton CT, US
International Classification:

G01N 33/53
US Classification:

435007100
Abstract:

The inventive subject matter relates to a competitive method measuring stress biomarkers in bodily fluids including serum, urine and oral fluids including saliva. The inventive method measures biomarkers including cortisol, melatonin and secretory IgA by fluorescence polarization fluorescence lifetime analysis or fluorescence resonance energy transfer.

Method For The Detection Of Target Molecules By Fluorescence Polarization Using Peptide Mimics
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US Patent:

20080227222, Sep 18, 2008
Filed:

Mar 13, 2007
Appl. No.:

11/726203
Inventors:

Malford E. Cullum - Grayslake IL, US
Karen H. O'Connor - Evanston IL, US
International Classification:

G01N 33/533
G01N 33/53
G01N 33/566
G01N 33/531
US Classification:

436546, 435 5, 435 72
Abstract:

The inventive subject matter relates to a method of measuring non-protein hydrophobic and protein target antigens by competitive fluorescence polarization using peptide mimics as competitors. The peptide mimics used in the inventive method contain conformational epitopes not represented in the linear sequence. The method can be used in the detection of a wide range of agents including steroids, hormones, nucleic acids, proteins and infectious organisms. A method for the detection of cortisol and gamma-interferon is also contemplated in the method. Samples suitable for analysis by the inventive method include environmental samples and bodily fluids including serum and oral fluids including saliva.

Reusable Urinary Catheter Products
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US Patent:

20220370760, Nov 24, 2022
Filed:

Sep 22, 2020
Appl. No.:

17/761970
Inventors:

- Libertyville IL, US
Horacio Montes de Oca Balderas - Ballina, IE
Malford E. Cullum - Grayslake IL, US
Varun Kumar - Dublin, IE
Ian Dodd - Ballina, IE
International Classification:

A61M 25/00
A61L 2/10
A61L 2/18
Abstract:

Reusable catheter products are provided for storing, transporting, and sterilizing reusable urinary catheters.