Nicholas De

US Patent:

56817460, Oct 28, 1997

Filed:

Dec 30, 1994

Appl. No.:

8/366851

Inventors:

Mordechai Bodner - San Diego CA
Nicholas J. De Polo - Solana Beach CA
Stephen Chang - Poway CA
David Chi-Tang Hsu - San Diego CA
James G. Respess - San Diego CA

Assignee:

Chiron Viagene, Inc.

International Classification:

C12N 500
C12N 1563
C07H 2104

US Classification:

435350

Abstract:

Retroviral vectors for directing expression of full length factor VIII in transduced host cells, plasmids encoding the same, and host cells transformed, transfected, or transduced therewith are disclosed. Also disclosed are retroviral particles comprising such retrovital vectors, as are methods for making such particles in suitable packaging cells. Retroviral particles so produced may be amphotropic, ecotropic, polytropic, or xenotropic; alternatively, they may comprise chimeric or hybrid envelope proteins to alter host range. Also described are retrovital particles comprising retroviral vectors for directing full length factor VIII expression which are complement resistant. Pharmaceutical compositions comprising retrovital particles of the invention are also disclosed, as are methods of treating mammals, particularly humans, afflicted with hemophilia.

US Patent:

45388504, Sep 3, 1985

Filed:

Mar 7, 1983

Appl. No.:

6/473102

Inventors:

Nicholas A. De Vito - Clifton NJ

International Classification:

B66C 134
B66C 166

US Classification:

294 89

Abstract:

A scissors-like engagement system includes pivotally secured first and second mating elements mutually including, at the ends of the mating elements, surfaces for complementally gripping and holding a projecting segment of an anchor element, in which the external surfaces of the ends of the mating elements define a parabolic surface nesting within a semi-hemispherical recess surrounding the anchor element. The complemental mating surfaces constitute male and female elements extending from, and centrally located within, the mating surfaces. The gripping surfaces are normally parallel to each other when the scissors-like engagement system is in a closed position about the anchor element. The male element of the complemental mating surfaces is an elongate, curvilinear, solid cylindrical member, the line of curvature of which lies equidistant from the surface of the semi-hemispherical recess within which the parabolic surface of the mating elements nest. The engagement system provides a positive interlock between the curvilinear male element and its complementary female element to, in combination with the anchor element gripping surfaces, limit movement of the anchor element relative to the engagement system, both within the linear axis of the male and female elements and in any transverse plane.