Richard S Joseph

US Patent:

20050208495, Sep 22, 2005

Filed:

Sep 17, 2003

Appl. No.:

10/528288

Inventors:

Richard Joseph - Stoughton MA, US
James DiMeo - Needham MA, US

International Classification:

C12Q001/68

US Classification:

435006000

Abstract:

A process is provided for using oligonucleotide to which a detectable moiety is attached post-synthesis. A metal-containing fluorescent compound affords real-time detection of nucleic acid elongation, amplification, or hybridization. The process is especially advantageous since a detectable moiety is readily attached to an existing oligonucleotide at an internal nucleotide, rather than being limited to attachment at a 3′ or 5′ terminus.

US Patent:

20060166215, Jul 27, 2006

Filed:

Jul 2, 2004

Appl. No.:

10/563347

Inventors:

Richard Joseph - Stoughton MA, US
James DiMeo - Needham MA, US

International Classification:

C12Q 1/68

US Classification:

435006000

Abstract:

A process for protecting a short RNA fragment includes labeling a short RNA fragment with a detectable platinum compound forming a labeled small RNA fragment. Resulting labeled short RNA fragment is exposed to a capture oligonucleotide. The capture oligonucleotide includes at least two replicates of a nucleotide sequence complimentary to the short RNA fragment nucleotide sequence. The labeled short RNA fragment and the captured oligonucleotide sequence are brought into contact under hybridization conditions. With hybridization, the marker moiety is detected on the hybridized labeled small RNA fragment-captured oligonucleotide conjugant. A detection array for short RNA fragment includes a substrate having multiple spots, with a first spot including a first capture oligonucleotide including at least two replicates of a nucleotide sequence complimentary to a first short RNA fragment along with an additional nucleotide sequence functioning as a universal controller or spacer. Another spot on the array includes a second capture oligonucleotide having at least two replicates of a sequence complimentary to a second short RNA fragment along with an additional sequence functioning as a universal controller or spacer. Verified small RNA fragments are also obtained after mobilizing a sequence through elution and optional platinum compound removal therefrom.

US Patent:

20100233696, Sep 16, 2010

Filed:

Nov 4, 2009

Appl. No.:

12/612520

Inventors:

Richard Joseph - Stoughton MA, US
James J. DiMeo - Needham MA, US
Mirna Jarosz - Arlington MA, US
John F. Thompson - Warwick RI, US
Jayson Bowers - Cambridge MA, US
Scott Chouinard - Medford MA, US
Philipp Kapronov - Cambridge MA, US
J. William Efcavitch - San Carlos CA, US
Christopher Hart - Sarasota FL, US
Fatih Ozsolak - Boston MA, US

Assignee:

HELICOS BIOSCIENCES CORPORATION - Cambridge MA

International Classification:

C12Q 1/68
B01L 3/00
G01N 33/53

US Classification:

435 6, 422102, 436501, 436 94

Abstract:

A method, flow cell and/or device for increasing the recovery of a limiting analyte in a sample, e.g., for single molecule analysis is disclosed. Methods for preparing a nucleic acid sample from a single cell and capturing nucleic acids on a surface configured for use in or with single molecule analysis are also provided.

US Patent:

20100233697, Sep 16, 2010

Filed:

Nov 13, 2009

Appl. No.:

12/618251

Inventors:

Richard Joseph - Stoughton MA, US
James J. DiMeo - Needham MA, US

Assignee:

HELICOS BIOSCIENCES CORPORATION - Cambridge MA

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

Methods are described which enable nucleic acid sample standardization prior to anchoring to a surface, especially useful in single molecule nucleic acid sequencing applications when sample is limiting or unamplified.

US Patent:

20120028822, Feb 2, 2012

Filed:

Nov 4, 2009

Appl. No.:

13/125932

Inventors:

Richard Joseph - Stoughton MA, US
James Dimeo - Needham MA, US
Mirna Jarosz - Arlington MA, US
John F. Thompson - Warwick RI, US
Jayson Bowers - Cambridge MA, US
Scott Chouinard - Medford MA, US
Philipp Kapranov - Sunnyvale CA, US
William J. Efcavitch - San Carlos CA, US
Christopher Hart - Cambridge MA, US
Fatih Ozsolak - Boston MA, US

Assignee:

HELICOS BIOSCIENCES CORPORATION - Cambridge MA

International Classification:

C40B 30/04
C40B 30/00
C12M 1/34
C12Q 1/68

US Classification:

506 9, 435 61, 506 7, 4352871

Abstract:

A method, flow cell and/or device for increasing the recovery of a limiting analyte in a sample, e.g., for single molecule analysis is disclosed. Methods for preparing a nucleic acid sample from a single cell and capturing nucleic acids on a surface configured for use in or with single molecule analysis are also provided.

Author:

Richard J. Joseph

ISBN #:

0815630212

Author:

Richard A. Joseph

ISBN #:

0521341361

Author:

Richard A. Joseph

ISBN #:

0793104505

Author:

Richard A. Joseph

ISBN #:

2865371573

Author:

Richard Joseph

ISBN #:

0131859226

Author:

Richard Joseph

ISBN #:

0131859463