Rui Hong

US Patent:

20130122516, May 16, 2013

Filed:

Jul 1, 2011

Appl. No.:

13/805983

Inventors:

Rui Hong - Oro Valley AZ, US
Hong Wang - Oro Valley AZ, US
Mark Lefever - Tucson AZ, US
Jan Froehlich - Oro Valley AZ, US
Christopher Bieniarz - Tucson AZ, US
Brian Kelly - Tucson AZ, US
Phillip Miller - Tucson AZ, US

International Classification:

G01N 27/62

US Classification:

435 71

Abstract:

Particular disclosed embodiments disclosed herein concern using a one or more various mass tags, which can be specifically deposited at targets through direct or indirect enzymatic-catalyzed transformation, to provide a method for identifying targets in tissue samples. The mass tags may be labeled with stable isotopes to produce mass tags having the same chemical structure but different masses. Mass codes produced by ionizing the mass tags are detected and/or quantified using mass spectrometry. The method can be used for multiplexed detection of multiple targets in a particular sample. In some embodiments, a map divided into sections representing sections of the tissue sample may be prepared, with the map sections including data corresponding to quantification data wherein the size of a mass peak is determined and correlated with the amount of a target for the corresponding tissue sample section.

US Patent:

20110124744, May 26, 2011

Filed:

Aug 15, 2008

Appl. No.:

12/673866

Inventors:

Lee Josephson - Reading MA, US
Rui Hong - Reading MA, US
Michael J. Cima - Winchester MA, US
Ralph Weissleder - Peabody MA, US

International Classification:

A61K 45/00
G01N 24/08

US Classification:

514789, 73 5441

Abstract:

This invention relates generally to magnetic resonance (MR)-based methods and kits for measuring the viscosity of liquid samples.

US Patent:

20210063411, Mar 4, 2021

Filed:

Sep 15, 2020

Appl. No.:

17/021659

Inventors:

- Tucson AZ, US
Jan Froehlich - Oro Valley AZ, US
Brian Daniel Kelly - Tucson AZ, US
Rui Hong - Oro Valley AZ, US
Mark Lefever - Oro Valley AZ, US
Phillip Miller - Tucson AZ, US
Hong Wang - Oro Valley AZ, US

International Classification:

G01N 33/68
G01N 27/62

Abstract:

Particular disclosed embodiments disclosed herein concern using a one or more various mass tags, which can be specifically deposited at targets through direct or indirect enzymatic-catalyzed transformation, to provide a method for identifying targets in tissue samples. The mass tags may be labeled with stable isotopes to produce mass tags having the same chemical structure but different masses. Mass codes produced by ionizing the mass tags are detected and/or quantified using mass spectrometry. The method can be used for multiplexed detection of multiple targets in a particular sample. In some embodiments, a map divided into sections representing sections of the tissue sample may be prepared, with the map sections including data corresponding to quantification data wherein the size of a mass peak is determined and correlated with the amount of a target for the corresponding tissue sample section.

US Patent:

20200271662, Aug 27, 2020

Filed:

Mar 4, 2020

Appl. No.:

16/809290

Inventors:

- Tucson AZ, US
Rui Hong - Oro Valley AZ, US

International Classification:

G01N 33/68
G01N 33/543
G01N 33/542

Abstract:

Methods for in situ detecting proximity of two targets of interest featuring an antibody conjugated with a cleavable bridge component having a detectable moiety and an antibody conjugated with a non-cleavable bridge component. The bridge components each have a chemical ligation group adapted to form a covalent bond under particular conditions and when the targets are in close proximity. Following covalent bond formation, the cleavable bridge component can be cleaved from the antibody, effectively transferring the detectable moiety to the non-cleavable bridge component. Detection of the detectable moiety is indicative of the targets being in close proximity. The methods are compatible with both chromogenic and fluorogenic detection systems. The methods may be used to perform assays wherein one or more than one proximity event is detected on the same slide.

US Patent:

20200095577, Mar 26, 2020

Filed:

Jun 11, 2019

Appl. No.:

16/438088

Inventors:

- Tucson AZ, US
Rui Hong - Oro Valley AZ, US

International Classification:

C12N 15/11
C07K 16/28
G01N 33/58
G01N 33/53
C07K 2/00
G01N 33/68
C07K 14/00

Abstract:

The disclosure is directed to conjugates, e.g. PNA conjugates, as well as methods of employing the conjugates for detecting one or more targets in a biological sample, e.g. a tissue sample.

US Patent:

20170254813, Sep 7, 2017

Filed:

May 23, 2017

Appl. No.:

15/603079

Inventors:

- Tucson AZ, US
Rui Hong - Oro Valley AZ, US

International Classification:

G01N 33/68
G01N 33/543

Abstract:

Methods for in situ detecting proximity of two targets of interest featuring an antibody conjugated with a cleavable bridge component having a detectable moiety and an antibody conjugated with a non-cleavable bridge component. The bridge components each have a chemical ligation group adapted to form a covalent bond under particular conditions and when the targets are in close proximity. Following covalent bond formation, the cleavable bridge component can be cleaved from the antibody, effectively transferring the detectable moiety to the non-cleavable bridge component. Detection of the detectable moiety is indicative of the targets being in close proximity. The methods are compatible with both chromogenic and fluorogenic detection systems. The methods may be used to perform assays wherein one or more than one proximity event is detected on the same slide.

US Patent:

20160002701, Jan 7, 2016

Filed:

Mar 11, 2014

Appl. No.:

14/769892

Inventors:

- Tucson AZ, US
Rui Hong - Oro Valley AZ, US
Zeyu Jiang - Tucson AZ, US

International Classification:

C12Q 1/25

Abstract:

A proximity detection method is described that utilizes enzymatic biotinylation to detect targets in a sample, particularly formalin fixed paraffin embedded samples using automated staining platforms. One disclosed embodiment comprises contacting the sample with a first conjugate comprising a biotin ligase and a first specific binding moiety that binds proximally to the first target; contacting the sample with a second conjugate comprising a biotin ligase substrate and a second specific binding moiety that binds proximally to the second target; subjecting the sample to conditions that allow biotinylation of the biotin ligase substrate by the biotin ligase when the first target and the second target have a proximal arrangement; and detecting biotinylation of the biotin ligase substrate. The conditions that allow biotinylation of the substrate include addition of biotin and ATP. The method also may comprise contacting the sample with a streptavidin-enzyme conjugate. Signal amplification also can be used.