Ezra Abrams - W. Newton MA, US Tianhong Zhang - Framingham MA, US Slawomir Mielewczyk - Newton MA, US Brian Patterson - Waltham MA, US
Assignee:
Matrix Technologies Corporation - Hudson NH
International Classification:
C12Q001/68 B05D003/00
US Classification:
435/006000, 427/002110
Abstract:
A method is provided for immobilizing a ligand, e.g., a nucleic acid, on a solid support. The method includes providing a solid support containing an immobilized latent thiol group, activating the thiol group, contacting the activated thiol group with a nucleic acid comprising an acrylamide functional group, and forming a covalent bond between the two groups, thereby immobilizing the nucleic acid to the solid support. Kits containing the solid supports and method of utilizing the solid supports are also provided.
Methods Of Immobilizing Ligands On Solid Supports And Apparatus And Methods Of Use Therefor
Ezra Abrams - W. Newton MA, US Tianhong Zhang - Framingham MA, US Slawomir Mielewczyk - Newton MA, US Brian Patterson - Waltham MA, US
Assignee:
Matrix Technologies Corporation - Hudson NH
International Classification:
C12Q001/68 B05D003/00
US Classification:
435/006000, 427/002110
Abstract:
A method is provided for immobilizing a ligand, e.g., a nucleic acid, on a solid support. The method includes providing a solid support containing an immobilized latent thiol group, activating the thiol group, contacting the activated thiol group with a nucleic acid comprising an acrylamide functional group, and forming a covalent bond between the two groups, thereby immobilizing the nucleic acid to the solid support. Kits containing the solid supports and method of utilizing the solid supports are also provided.
A method is provided for immobilizing a ligand, e. g. , a nucleic acid, on a solid support. The method includes providing a solid support containing an immobilized latent thiol group, activating the thiol group, contacting the activated thiol group with a nucleic acid comprising an acrylamide functional group, and forming a covalent bond between the two groups, thereby immobilizing the nucleic acid to the solid support. Kits containing the solid supports and method of utilizing the solid supports are also provided.