Tatsuro Irimura

US Patent:

53328124, Jul 26, 1994

Filed:

Oct 15, 1991

Appl. No.:

7/776691

Inventors:

Garth L. Nicolson - Kingswood TX
Motowo Nakajima - Houston TX
Tatsuro Irimura - Bellaire TX

Assignee:

Board of Regents, The University of Texas System - Austin TX

International Classification:

C07H 100
C08B 3710

US Classification:

536 21

Abstract:

A solid phase substrate which yields soluble labeled products upon hydrolysis by a glycosaminoglycan endoglycosidase and methods of producing said substrate are comprised in the present invention. The solid phase substrate comprises glycosaminoglycan bearing labeled substances bound to amino groups. The labeled glycosaminoglycan substrate is reductively aminated at its reducing terminal end to produce an amine-terminus. The substrate is further coupled to an amino-reactive solid matrix through its amine-terminus.

US Patent:

48595810, Aug 22, 1989

Filed:

Feb 20, 1987

Appl. No.:

7/012860

Inventors:

Garth L. Nicolson - Kingwood TX
Motowo Nakajima - Houston TX
Tatsuro Irimura - Bellaire TX

Assignee:

Board of Regents, The University of Texas System - Austin TX

International Classification:

C12Q 100

US Classification:

435 4

Abstract:

A solid phase substrate which yields soluble labeled products upon hydrolysis by a glycosaminoglycan endoglycosidase and methods of producing said substrate are comprised in the present invention. The solid phase substrate comprises glycosaminoglycan bearing labeled substances bound to amino groups. The labeled glycosaminoglycan substrate is reductively aminated at its reducing terminal end to produce an amine-terminus. The substrate is further coupled to an amino-reactive solid matrix through its amine-terminus. A method of producing the solid phase substrate comprises the steps of: at least partially N-desulfating or N-deacylating a glycosaminoglycan; labeling at least partially N-deacylated or N-desulfated glycosaminoglycan with a substance yielding detectable signals to produce labeled glycosaminoglycan; completely N-acylating the labeled glycosaminoglycan with acyl anhydride or acyl halide; reductively aminating a reducing terminal end of said labeled glycosaminoglycan to produce labeled amine-terminal glycosaminoglycan; and coupling, through its terminal amine, the labeled amine-terminal glycosaminoglycan to an amino-reactive solid phase support to produce the solid phase substrate. The solid phase substrate is usable to detect metastatic tumors by measurement of serum heparanase levels. The potential metastases of a tumor may also be determined by its heparanase levels.