Thomas W Schoenfeld

age ~62

from Topsfield, MA

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Thomas W Shoenfeld

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Thomas Schoenfeld Phones & Addresses

Topsfield, MA
Milwaukee, WI
5310 Regent St, Madison, WI 53705 • 6082331280

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Phones & Addresses

Thomas R. Schoenfeld

MAJOR MOVES, LLC

Us Patents

Thermostable Viral Polymerases And Methods Of Use
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US Patent:

8093030, Jan 10, 2012
Filed:

Oct 6, 2006
Appl. No.:

12/089221
Inventors:

Thomas W. Schoenfeld - Madison WI, US
Vinay K. Dhodda - Madison WI, US
Robert A. Difrancesco - Waunakee WI, US
David A. Mead - Middleton WI, US
Assignee:

Lucigen Corporation - Middleton WI
International Classification:

C12N 9/12
C12P 19/34
US Classification:

435194, 435183, 435 911, 530350
Abstract:

Thermostable viral polymerases exhibiting a combination of activities selected from, proofreading (3′-5′) exonuclease activity, nick translating (5′-3′) nuclease activity, synthetic primer-initiated polymerase activity, nick-initiated polymerase activity, reverse transcriptase activity, strand displacement activity, and/or decreased discrimination against incorporation of nucleotide analogs. Also provided are compositions including the polymerases, polynucleotides encoding the polymerases and methods of using the polymerases.

Viral Libraries From Uncultivated Viruses And Polypeptides Produced Therefrom
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US Patent:

20050282155, Dec 22, 2005
Filed:

Jun 15, 2005
Appl. No.:

11/152883
Inventors:

Thomas Schoenfeld - Madison WI, US
David Mead - Middleton WI, US
Assignee:

Lucigen Corporation - Middleton WI
International Classification:

C12Q001/70
C12Q001/68
US Classification:

435005000, 435006000
Abstract:

Provided is a method for producing viral genomic and complementary DNA expression libraries and novel viral polypeptides without requiring virus cultivation prior to library construction. The method includes direct isolation of viral particles from the environment by differential filtration and centrifugation. The viral nucleic acids are extracted and used to construct libraries that may be screened by one of several methods to identify useful coding sequences or may be used to produce novel polypeptides.

Vectors, Kits And Methods For Cloning Dna
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US Patent:

20080166773, Jul 10, 2008
Filed:

Jan 8, 2007
Appl. No.:

11/621031
Inventors:

David A. Mead - Middleton WI, US
Ronald Godiska - Verona WI, US
Thomas W. Schoenfeld - Madison WI, US
Spencer Hermanson - Watertown WI, US
Assignee:

LUCIGEN CORPORATION - Middleton WI
International Classification:

C07H 21/04
C12N 15/64
US Classification:

435 914, 4353201, 536 242
Abstract:

Vector preparations and cloning constructs suitable for use in cloning are provided. Vector preparations are double-stranded DNA molecules having two 3′ termini, each terminus having a single base pair overhang that is capable of hybridizing to a single base pair overhang on a double stranded polynucleotide sequence to be cloned. The overhang of the vector preparation is suitably a dCMP and the overhang of the polynucleotide sequence to be cloned is suitably a dGMP. In other embodiments, the overhang of the polynucleotide sequence to be cloned is any ddNTP and the corresponding overhang of the vector preparation is any base that pairs to the ddNTP. The latter embodiment is particularly suited to preparing recombinant molecules having only a single insert. Methods of cloning, methods of preparing libraries of recombinant molecules and kits for carrying out the methods are also provided.

Thermostable Dna Polymerases And Methods Of Use
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US Patent:

20100291638, Nov 18, 2010
Filed:

Apr 15, 2010
Appl. No.:

12/761175
Inventors:

Thomas William Schoenfeld - Madison WI, US
Nicholas Hermersmann - Beaver Dam WI, US
Darby Renneckar - Madison WI, US
David Alan Mead - Middleton WI, US
International Classification:

C12P 19/34
C12N 9/10
C07H 21/04
C12N 1/21
US Classification:

435 9151, 435193, 536 232, 43525233, 435 915
Abstract:

Thermostable viral and microbial polymerases exhibiting a combination of activities selected from proofreading (3′-5′) exonuclease activity, nick translating (5′-3′) nuclease activity, synthetic primer-initiated polymerase activity, nick-initiated polymerase activity, reverse transcriptase activity, strand displacement activity, terminal transferase activity, primase activity, and/or efficient incorporation of chain terminating analogs. Some of the polymerases provided herein include a first motif and a second motif. The first motif preferably has the sequence XXXDXPXIELRXXX, wherein Xis I or V; Xis F or Y; Xis G or A; and X, X, X, X, and Xare any amino acid. The second motif preferably has the sequence RXXXKSANXGXXYG, wherein Xis G or A; Xis F, L, or Y; Xis L or V; Xis I or L; and Xand Xare any amino acid. Also provided are reagents for expressing the polymerases, including polynucleotides encoding the polymerases and host cells expressing the polymerases, and methods of using the polymerases.

Thermostable Dna Polymerases And Methods Of Use
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US Patent:

20120083018, Apr 5, 2012
Filed:

Dec 7, 2011
Appl. No.:

13/313783
Inventors:

Thomas W. Schoenfeld - Madison WI, US
David A. Mead - Middleton WI, US
International Classification:

C12P 19/34
C12N 9/12
US Classification:

435 9151, 435194, 435 915
Abstract:

Thermostable viral and microbial polymerases exhibiting a combination of activities selected from proofreading (3′-5′) exonuclease activity, nick translating (5′-3′) nuclease activity, synthetic primer-initiated polymerase activity, nick-initiated polymerase activity, reverse transcriptase activity, strand displacement activity, terminal transferase activity, primase activity, and/or efficient incorporation of chain terminating analogs. Some of the polymerases provided herein include a first motif and a second motif. The first motif preferably has the sequence XXXDXPXIELRXXX, wherein Xis I or V; Xis F or Y; Xis G or A; and X, X, X, X, and Xare any amino acid. The second motif preferably has the sequence RXXXKSANXGXXYG, wherein Xis G or A; Xis F, L, or Y; Xis L or V; Xis I or L; and Xand Xare any amino acid. Also provided are reagents for expressing the polymerases, polynucleotides encoding the polymerases, host cells expressing the polymerases, and methods of using the polymerases.

Rna- And Dna-Copying Enzymes
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US Patent:

20130022980, Jan 24, 2013
Filed:

Feb 4, 2010
Appl. No.:

13/147446
Inventors:

Robert Michael Nelson - Wellesley MA, US
Thomas W. Schoenfeld - Madison WI, US
David A. Mead - Middleton WI, US
International Classification:

C12N 9/96
C12Q 1/68
C07H 21/04
C12P 19/34
US Classification:

435 612, 435188, 435 915, 435 61, 536 232, 435 912
Abstract:

The present invention is directed to DNA polymerase fusion proteins with increased processivity and nucleic acid affinity. The invention includes a fusion protein comprising a nucleic acid-binding domain fused to a polymerase domain. The nucleic acid binding domain contains at least one nucleic acid binding motif, such as a DNA-binding motif or an RNA-binding motif. The nucleic acid binding domain preferably embodies an oligonucleotide/oligosaccharide binding (OB) fold, among other conformations. The invention further includes methods of synthesizing nucleic acids using the fusion proteins described herein.

Improved Thermostable Viral Reverse Transcriptase
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US Patent:

20220348891, Nov 3, 2022
Filed:

Sep 18, 2020
Appl. No.:

17/761053
Inventors:

- Bevelry MA, US
David Mark SCHUSTER - Poolesville MD, US
Thomas William SCHOENFELD - Topsfield MA, US
International Classification:

C12N 9/12
Abstract:

The present invention provides novel engineered reverse transcriptase enzymes that afford beneficial improvements in thermal stability, processivity, cDNA yields and elimination of secondary enzymatic activity. The present invention also provides methods for amplifying template nucleic acids using such reverse transcriptase enzymes. This invention addresses deficiencies in the current state of the art reverse transcriptase enzymes in RNA detection and analysis including deficiencies in detection sensitivity, specificity, side enzyme activities, enzyme stability and synthesis capacity, especially when using template nucleic acids ranging in length, secondary structure and nucleotide content.

Molecular Detection Of Rna
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US Patent:

20160348189, Dec 1, 2016
Filed:

May 27, 2016
Appl. No.:

15/166497
Inventors:

- Middleton WI, US
Thomas William Schoenfeld - Topsfield MA, US
Assignee:

LUCIGEN CORPORATION - Middleton WI
International Classification:

C12Q 1/70
C12Q 1/68
Abstract:

Methods of detecting RNA, such as ribosomal RNA (rRNA), messenger RNA (mRNA), and others. The methods include heating a cell comprising RNA in a solution to release the RNA from the cell, reverse transcribing the RNA into DNA with an enzyme, amplifying the DNA with the same enzyme, and detecting the amplified DNA. The heating, reverse-transcribing, and amplifying in at least some of the methods are performed at substantially the same temperature and a substantially constant temperature without adding additional reagents during or between the steps. The methods can be used to detect the presence of one cell type as distinguished from another cell type within a sample or to determine levels of gene expression, each without the need for elaborate extraction protocols.