Tina C Kuhlmann

US Patent:

20050081268, Apr 14, 2005

Filed:

Apr 8, 2002

Appl. No.:

10/473945

Inventors:

Steve Kay - San Diego CA, US
Tina Kuhlmann - San Diego CA, US
Richard Lerner - La Jolla CA, US

International Classification:

A01H001/00
C12N015/82
C12N005/04

US Classification:

800288000, 435419000

Abstract:

A genetically modified plant cell containing a heterologous nucleotide sequence encoding a bioluminescent polypeptide, which is expressed in amount sufficient to produce at least about 750,000 photons of visible light/mm/second, is provided, as is a visibly bioluminescent transgenic plant, which contains such a genetically modified plant cell. Also provided is a recombinant nucleic acid molecule, which contains a plant translational enhancer operatively linked to a nucleotide sequence encoding a bioluminescent polypeptide. In addition, methods of producing a genetically modified plant cell that is visibly bioluminescent introducing a transgene encoding a bioluminescent polypeptide into a plant cell, whereby the bioluminescent polypeptide is expressed at a level that produces at least about 750,000 photons of visible light/mm/second are provided, as are kits containing such visibly bioluminescent compositions.