William J Biddle

US Patent:

6383810, May 7, 2002

Filed:

Feb 13, 1998

Appl. No.:

09/023790

Inventors:

Richard Fike - Clarence NY
William Whitford - Santa Fe NM
William Biddle - Buffalo NY

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N 500

US Classification:

435384, 435243, 4352536, 43525521, 4352568, 435325, 435404, 435410, 435431

Abstract:

The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention is particularly directed to methods of production of these media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. The invention also relates to methods of producing sterile powdered media, media supplement (particularly powdered sera such as powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts (such as bovine brain or pituitary extracts), powdered growth factors (such as EGF, FGF, etc. ) and the like), media subgroup and buffer formulations.

US Patent:

6627426, Sep 30, 2003

Filed:

May 23, 2000

Appl. No.:

09/576900

Inventors:

William C. Biddle - Buffalo NY
Richard M. Fike - Clarence NY
Barbara M. Dadey - East Aurora NY
Thomas E. Bulera - Lancaster NY

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N 100

US Classification:

435243, 435252, 435254, 435325, 435355, 435420

Abstract:

The present invention relates generally to a method to reduce, substantially reduce, inactivate or eliminate adventitious agents and/or toxins in a sample, particuarly in nutritive media, media supplements, media subgroups and buffer formulations. Specifically, the present invention provides powdered nutritive media, media supplements and media subgroups produced by the methods of the invention, particuarly cell culture media supplements (including powdered sera such as powdered fetal bovine serum (FBS)). The invention further provides powdered buffer formulations produced by the methods of the invention. The invention also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these nutritive media, media supplements, media subgroups and buffer formulations. The invention also relates to methods for producing storage stable cells having reduced, substantially reduced or eliminated adventitious agents or toxins.

US Patent:

6733746, May 11, 2004

Filed:

Jan 31, 1997

Appl. No.:

08/792299

Inventors:

John P. Daley - Buffalo NY
Barbara M. Dadey - East Aurora NY
William C. Biddle - Buffalo NY
Michelle G. Wysocki - Cheektowaga NY

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

A01N 6300

US Classification:

424 9321, 424 931, 424 932, 435372, 435383, 435384, 435386, 435387, 435388, 435389, 435404, 435405, 435406, 435407

Abstract:

The present invention provides a serum-free supplement which supports the growth of hematopoietic cells in culture. Also provided are a medium comprising a basal medium supplemented with the serum-free supplement of the present invention. The present invention also provides methods for culturing and for differentiating hematopoietic cells.

US Patent:

7572632, Aug 11, 2009

Filed:

Oct 16, 2003

Appl. No.:

10/685802

Inventors:

Richard Fike - Clarence NY, US
William Whitford - Santa Fe NM, US
William Biddle - Buffalo NY, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12N 6/00
C12N 1/00
C12N 1/20
C12N 5/00

US Classification:

435384, 435243, 4352536, 43525521, 4352568, 435325, 435404, 435431

Abstract:

Methods and compositions involving nutritive media, media supplements, media subgroup and buffer formulations are provided. Powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent are also disclosed. Methods of producing these media, media supplements, media subgroups and buffer formulations as well as kits and methods for cultivation of procaryotic and eukaryotic cells (including human cells) are also provided. Also disclosed are methods of producing sterile powdered media, media supplements, powdered growth factors, media subgroup and buffer formulations. Methods of sterilization include gamma irradiation. Methods for producing dry cell powders where spray-drying a cell suspension may be used are disclosed. Cells, media, media supplements, media subgroups and buffer powders produced by these methods are also disclosed.

US Patent:

20040022666, Feb 5, 2004

Filed:

Jul 11, 2003

Appl. No.:

10/617377

Inventors:

William Biddle - Buffalo NY, US
Richard Fike - Clarence NY, US
Barbara Dadey - East Aurora NY, US
Thomas Bulera - Lancaster NY, US

Assignee:

Invitrogen Corporation

International Classification:

G05B001/00

US Classification:

422/003000

Abstract:

The present invention relates generally to a method to reduce, substantially reduce, inactivate or eliminate adventitious agents and/or toxins in a sample, particuarly in nutritive media, media supplements, media subgroups and buffer formulations. Specifically, the present invention provides powdered nutritive media, media supplements and media subgroups produced by the methods of the invention, particuarly cell culture media supplements (including powdered sera such as powdered fetal bovine serum (FBS)). The invention further provides powdered buffer formulations produced by the methods of the invention. The invention also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these nutritive media, media supplements, media subgroups and buffer formulations. The invention also relates to methods for producing storage stable cells having reduced, substantially reduced or eliminated adventitious agents or toxins.

US Patent:

20040072349, Apr 15, 2004

Filed:

Nov 20, 2003

Appl. No.:

10/716619

Inventors:

John Daley - Buffalo NY, US
Barbara Dadey - East Aurora NY, US
William Biddle - Buffalo NY, US
Michelle Wysocki - Cheektowaga NY, US

Assignee:

Invitrogen Corporation

International Classification:

C12N005/02

US Classification:

435/407000

Abstract:

The present invention provides a serum-free supplement which supports the growth of hematopoietic cells in culture. Also provided are a medium comprising a basal medium supplemented with the serum-free supplement of the present invention. The present invention also provides methods for culturing and for differentiating hematopoietic cells.

US Patent:

20060003447, Jan 5, 2006

Filed:

Dec 29, 2004

Appl. No.:

11/024051

Inventors:

Richard Fike - Clarence NY, US
William Whitford - Santa Fe NM, US
William Biddle - Buffalo NY, US
Laurel Biddle - Buffalo NY, US
Christine Biddle - Buffalo NY, US
Jeffrey Biddle - Buffalo NY, US

International Classification:

C12N 5/02

US Classification:

435404000

Abstract:

The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.

US Patent:

20060003448, Jan 5, 2006

Filed:

Dec 29, 2004

Appl. No.:

11/024053

Inventors:

Richard Fike - Clarence NY, US
William Whitford - Santa Fe NM, US
William Biddle - Buffalo NY, US
Laurel Biddle - Buffalo NY, US
Christine Biddle - Buffalo NY, US
Jeffrey Biddle - Buffalo NY, US
Richard Hassett - Tonewanda NY, US
Barbara Dadey - East Aurora NY, US

International Classification:

C12N 5/02

US Classification:

435404000

Abstract:

The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.

Author:

William A. Biddle

ISBN #:

0738542040