Yan Qiu Wu

US Patent:

20050180809, Aug 18, 2005

Filed:

Feb 13, 2004

Appl. No.:

10/779095

Inventors:

Yan Wu - Glenview IL, US
Richard Maddrell - Barrington IL, US

Assignee:

Publications International, Ltd. - Lincolnwood IL

International Classification:

B42D003/00

US Classification:

402070000

Abstract:

A binding system comprising a spine adapted to be removeably secured within a base and a clip adapted to connect to the spine. The clip may be threaded through the aperture of a leaf to movably secure the leaf to the spine. Further, the spine may be elongated with a pair of mushroom-shaped ends. The base may further include a well having end walls with opposed dimples. The mushroom shaped heads may fit within the dimples to secure the spine to the base. The spine may include an aperture into which at least a portion of the clip may be inserted to secure the clip to the spine. The at least one leaf may be permitted to lie substantially flat upon the base when the spine is secured within the well.

US Patent:

D4361008, Jan 9, 2001

Filed:

Jul 8, 1999

Appl. No.:

D/107582

Inventors:

Ann Taylor - Winnetka IL
Yan Wu - Glenview IL

Assignee:

Publications International, Ltd. - Lincolnwood IL

International Classification:

1401

US Classification:

D14226

US Patent:

20220235104, Jul 28, 2022

Filed:

Jul 3, 2019

Appl. No.:

17/257951

Inventors:

- Oakland CA, US
Yan Wu - La Jolla CA, US
Amir Dailamy - La Jolla CA, US
Prashant Mali - La Jolla CA, US
Daniella McDonald - La Jolla CA, US
Udit Parekh - La Jolla CA, US
Michael Hu - La Jolla CA, US

International Classification:

C07K 14/47
A61K 31/522
A61K 45/06
C12N 15/86
C12N 5/071
C12N 15/11
C12N 9/22

Abstract:

This disclosure relates to methods, polynucleotides, vectors, viral particles, cells, and systems or the engineering of human tissues. One aspect of the disclosure relates to using lineage-specific miRNA binding molecules to bias tissue lineage. Another aspect of the disclosure relates to using lineage-specific transcription factor overexpression to bias tissue lineage.

US Patent:

20210108193, Apr 15, 2021

Filed:

Sep 22, 2020

Appl. No.:

17/028836

Inventors:

- Oakland CA, US
Udit Parekh - La Jolla CA, US
Yan Wu - La Jolla CA, US
Kun Zhang - La Jolla CA, US

International Classification:

C12N 15/10
C12N 5/071
C12N 15/86
A61K 35/44

Abstract:

Understanding the complex effects of genetic perturbations on cellular state and fitness in human pluripotent stem cells (hPSCs) has been challenging using traditional pooled screening techniques which typically rely on unidimensional phenotypic readouts. Here, Applicants use barcoded open reading frame (ORF) overexpression libraries with a coupled single-cell RNA sequencing (scRNA-seq) and fitness screening approach, a technique we call SEUSS (ScalablE fUnctional Screening by Sequencing), to establish a comprehensive assaying platform. Using this system, Applicants perturbed hPSCs with a library of developmentally critical transcription factors (TFs), and assayed the impact of TF overexpression on fitness and transcriptomic cell state across multiple media conditions. Applicants further leveraged the versatility of the ORF library approach to systematically assay mutant gene libraries and also whole gene families. From the transcriptomic responses, Applicants built genetic co-perturbation networks to identify key altered gene modules. Strikingly, we found that KLF4 and SNAI2 have opposing effects on the pluripotency gene module, highlighting the power of this method to characterize the effects of genetic perturbations. From the fitness responses, Applicants identified ETV2 as a driver of reprogramming towards an endothelial-like state.

US Patent:

20190330661, Oct 31, 2019

Filed:

Jun 28, 2017

Appl. No.:

16/312907

Inventors:

- Oakland CA, US
Prashant MALI - La Jolla CA, US
Yan WU - La Jolla CA, US
Dongxin ZHAO - La Jolla CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 15/90
C12N 15/10
C12N 9/22
G16B 20/20
G16B 25/10
G16B 40/10
C12N 15/113

Abstract:

A guide RNA comprising: a gRNA spacer sequence at the 5′ end of the guide RNA, wherein the spacer sequence is complementary to a target gene, a scaffold sequence that binds to Cas9, and an RNA capture and sequencing domain comprising: a barcode sequence, and a primer binding sequence; nucleic acids and vectors encoding the guide RNA; cells expressing the guide RNA; and a library comprising a plurality of guide RNAs. Also disclosed are methods of introducing a genetic perturbation into a cell, methods of assessing an effect of at least one genetic perturbation on RNA expression in a cell, methods of identifying nucleic acid sequences associated with a disease state and a method of identifying candidate therapeutic agents.

Author:

Yan Wu

ISBN #:

7532848027