Zhiwu Wu Lin

US Patent:

20020146679, Oct 10, 2002

Filed:

Dec 18, 2001

Appl. No.:

10/025591

Inventors:

Sotirios Karathanasis - Saline MI, US
Zhiwu Lin - Ann Arbor MI, US
Robert Panek - Ann Arbor MI, US

International Classification:

A61K038/19
C12Q001/00
C12Q001/68

US Classification:

435/004000, 435/006000, 424/085100

Abstract:

The proposed invention relates to a method for creating cell culture conditions which causes human endothelial cells in culture when exposed to a cytokine, preferably, TNF for periods of longer than 5 days (chronic exposure) to become unresponsive to growth factors and other substances in serum. The cell culture assay system allows for screening for novel molecules that may correct endothelial dysfunction and will help identify genes that could be used to prevent or correct endothelial dysfunction with gene therapy.